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The Effect of Red Cell Aging on Chromium-51 Binding and in Vitro Elution¹

Alexandria, Egypt, U.A.R.

ABSTRACT

Differential osmotic lysis and centrifugional separation of red cells were used to study the relationship between red cell age and ⁵¹Cr-hemoglobin binding and in in vitro elution of the label during electrophoresis.

The hemoglobin specific activity was uniform in hemolysates obtained by graded saline hemolysis except for the 1.0% most fragile cells, which showed a higher specific activity. The previously reported higher specific activity of the top cells separated by centrifugation was also shown. Labeling of red cells with radiochromate did not alter the pattern of osmotic fragility and density relationship between old and young cells.

The elution of label during electrophoresis was significantly higher from solutions obtained from old cells than from that of relatively young cells. Washing of cells with phosphate buffered ACD markedly reduced the elution of label. Artificial induction of increased fragility resulted in a marked increase in the rate of elution.

The significance of these findings in the interpretation of in vivo ⁵¹Cr-labeled red cell survival curves is discussed.

FOOTNOTES

¹ Work done in the Radioisotopes Unit of the Medical Research Institute, Alexandria.

² From the Department of Radiology, Faculty of Medicine, University of Alexandria. Present Address: The Harvard Medical School, Department of Radiology at the Peter Bent Brigham Hospital, Boston, Mass.

³ From the Radioisotopes Unit of the Medical Research Institute.