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Factors Influencing Dextran-Gel Filtration of Serum and Iodine-131 Labelled L-Thyroxine

Charlottesville, Virginia

ABSTRACT

Non-thyroid factors affecting the dissociation of ¹³¹I thyroxine from serum during dextran-gel chromatography have been analysed. The important variables are the type of gel, dimensions and temperature of, and flow rate through, the gel bed. Their regulation is mandatory when the technique is used in the assessment of thyroid function, and when attained, the reproducibility is satisfactory.

FOOTNOTES

¹ United States Public Health Service Postdoctoral Fellow, National Heart Institute.

² Department of Medicine, University of Newcastle upon Tyne, Newcastle upon Tyne, England.

³ Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia.

⁴ Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville, Virginia.