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The Determination of Protein Bound Iodine-131 In Thyroid Gland Homogenates

Detroit, Michigan

ABSTRACT

A comparison has been made between filter-paper electrophoresis and chromatography in an acid butanol system for the determination of ¹³¹I bound to protein in rat thyroid homogenates. Correlation between the two methods over a wide range of values was quite good (r = .973). However, electrophoresis may be preferred by some investigators because it requires less time. Chromatography of thyroid extracts in butanol:dioxane:ammonia was not very satisfactory since the value for ¹³¹PBI was uniformly lower when compared to chromatograms developed in acid butanol.

FOOTNOTES

¹ Woman's Hospital, Detroit, Michigan 48201.