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Determination of the Amino Acid Active Transport into Tissue Cells^1,2,

Chicago, Illinois

ABSTRACT

The described procedure allows the determination of the volume of the intracellular and extracellular water compartments on the same tissue sample as that used to determine the activity of the transported substance (in this case AIB-¹⁴C). It therefore permits a more accurate measurement of active transport than by using separate tissue specimens for these determinations. This procedure has also solved many of the technical problems involved in working with very small specimens of fresh tissue. With this procedure it has been possible to measure the active cellular transport of AIB-¹⁴C in specimens as small as 0.2 mg.

FOOTNOTES

¹ From the Radioisotope Service and Medical Service, Veterans Administration West Side Hospital; and the Department of Medicine, University of Illinois, College of Medicine, Chicago.

² Presented at the 11th Annual Meeting of The Society of Nuclear Medicine, Berkeley, California, June 17–20, 1964.