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Studies of In Vitro Incorporation of p³² By Human Erythrocytes^1,2,3,

Hines, Illinois

ABSTRACT

1. For optimal P³² uptake, the ratio of blood to beaker volume is critical, and should be about 1:10.

2. Increasing the shaking rate of a blood sample incubated in a nonoptimal beaker size, increases the P³² uptake.

3. Incubation of erythrocytes in a saline medium containing P³² produces a significant increase in P³² uptake when compared to the uptake in a plasma medium.

4. As the concentration of inorganic phosphate is increased in a saline medium containing erythrocytes, the P³² uptake by the cells is decreased.

5. No appreciable differences in P³² uptake were noted when the following experiments were performed: (1) Anaerobic vs. aerobic incubation; (2) Heparin levels (0.05 mg/ml blood to 1.00 mg/ml blood); (3) Paraffin-coated beaker vs. glass beaker; (4) Subjecting red cells to centrifugational speeds of 270 xg and 7,710 xg prior to incubation with P³².

FOOTNOTES

¹ From the Stritch School of Medicine, Loyola University, Chicago, and Veterans Administration Hospital, Hines, Illinois.

² This investigation was done during a clinical investigatorship in Veterans Administration Hospital, Hines, Illinois.

³ This investigation was supported in part by a grant from the Psychiatric Training and Research Fund of the Illinois Department of Mental Health.