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The Journal of Nuclear Medicine Vol. 5 No. 4 304-306
© 1964 by Society of Nuclear Medicine
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Intermolecular Reactions between Glycerol and Lactate Dehydrogenase. Influence on the Radiation Sensitivity of the Enzyme1

W. Lohmann, Ph.D., A. J. Moss, Jr., B.S. and W. H. Perkins, M.D.

Little Rock, Arkansas

ABSTRACT

The change in radiosensitivity of lactate dehydrogenase by glycerol was investigated spectrophotometrically. The irradiation doses varied between 0 to 4.5 x 105 r. Small amounts of glycerol (0.0005 vol.%) gave a maximum protective effect. This protective effect can be explained by a complex formation between glycerol and the metal ions present in the enzyme molecule.

FOOTNOTES

1 Departments of Physiology, Radiology, and Medicine, University of Arkansas Medical Center; and VA Research Support Center, Little Rock, Arkansas.







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Copyright © 1964 by the Society of Nuclear Medicine.