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The Journal of Nuclear Medicine Vol. 5 No. 4 297-301
© 1964 by Society of Nuclear Medicine
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Determination of Citric Acid in Serum and Urine Using Br82

S. L. Jacobs, Ph. D.1 and Norman D. Lee, Ph. D.

Los Angeles

ABSTRACT

Radiometric assay for Br82 was used in the analysis for citric acid by incorporation of this isotope in the brominating reagent for conversion of the citric acid to pentabromoacetone. This provided a rapid and simple means for quantitative recovery of added citric acid and a precision (95% confidence limits) of ± 9.2 per cent and ± 6.6 per cent was obtained for serum and urine specimens, respectively. Results by this technic were slightly higher for urine than those obtained by the photometric method used for comparison, whereas results for serum agreed well.

FOOTNOTES

1 Bio-Science Laboratories, Los Angeles 25, Calif.






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Copyright © 1964 by the Society of Nuclear Medicine.