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First published online May 15, 2008, 10.2967/jnumed.108.050880
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Journal of Nuclear Medicine Vol. 49 No. 6 995-999
© 2008 by Society of Nuclear Medicine
doi: 10.2967/jnumed.108.050880

Basic Science Investigation

Comparison of 99mTc- and 18F-Ubiquicidin Autoradiography to Anti–Staphylococcus aureus Immunofluorescence in Rat Muscle Abscesses

Dagmar Salber1, Johannes Gunawan2, Karl-Josef Langen3, Eva Fricke2, Peter Klauth4, Wolfgang Burchert2 and Sijtse Zijlstra2

1 C. & O. Vogt Institute of Brain Research, University Hospital Düsseldorf, Düsseldorf, Germany; 2 Institute of Radiology, Nuclear Medicine, and Molecular Imaging, Heart and Diabetes Centre North Rhine–Westphalia, Bad Oeynhausen, Germany; 3 Institute of Neuroscience and Biophysics, Medicine Research Center Jülich, Jülich, Germany; and 4 Institute of Chemistry and Dynamics, Geosphere-Agrosphere Research Center, Jülich, Germany

Correspondence: For correspondence or reprints contact: Karl-Josef Langen, Institute of Neuroscience and Biophysics–Medicine, Research Center Jülich, D-52425 Jülich, Germany. E-mail: k.j.langen{at}fz-juelich.de

99mTc-ubiquicidin (UBI) 29-41 is under clinical evaluation for discrimination between bacterial infection and unspecific inflammation. We compared the distribution of 99mTc-UBI 29-41, the potential PET tracers 18F-UBI 29-41 and 18F-UBI 28-41, and 3H-deoxyglucose (DG) in rat muscle abscesses to that of anti–Staphylococcus aureus immunofluorescent imaging. Methods: Calf abscesses were induced in 15 CDF-Fischer rats after inoculation of Staphylococcus aureus. One to 6 d later, either 18F-UBI 29-41 and 3H-DG (n = 5) or 18F-UBI 28-41 and 3H-DG (n = 6) or 99mTc-UBI 29-41 and 3H-DG (n = 4) were injected simultaneously. Dual-tracer autoradiography of the abscess area was compared with the distribution of bacteria and macrophages. Results: The UBI derivates exhibited increased uptake in the abscess area that partly matched 3H-DG uptake and macrophage infiltration but showed no congruity with areas that were highly positive for bacteria. Conclusion: A specific binding of UBI derivatives to Staphylococcus aureus in vivo could not be confirmed in this study.

Key Words: bacterial infections • ubiquicidin • 99mTc-UBI 29-41 • 18F-UBI 29-41 • 18F-UBI 28-41 • autoradiography • S. aureus

COPYRIGHT © 2008 by the Society of Nuclear Medicine, Inc.


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