JNM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online September 15, 2008, 10.2967/jnumed.108.054361
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jnumed.108.054361v1
49/10/1701    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JNM
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kusmic, C.
Right arrow Articles by Sambuceti, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kusmic, C.
Right arrow Articles by Sambuceti, G.
Journal of Nuclear Medicine Vol. 49 No. 10 1701-1706
© 2008 by Society of Nuclear Medicine
doi: 10.2967/jnumed.108.054361

Basic Science Investigation

Whole-Body Evaluation of MIBG Tissue Extraction in a Mouse Model of Long-Lasting Type II Diabetes and Its Relationship with Norepinephrine Transport Protein Concentration

Claudia Kusmic1, Silvia Morbelli2, Cecilia Marini1, Marco Matteucci3, Chiara Cappellini3, Elena Pomposelli2, Paolo Marzullo1, Antonio L'Abbate1,3 and Gianmario Sambuceti2

1 Institute of Clinical Physiology CNR, Pisa, Italy 2 Department of Internal Medicine, Division of Nuclear Medicine, University of Genoa, Genova, Italy; and 3 Scuola Superiore Sant'Anna, Pisa, Italy

Correspondence: For correspondence or reprints contact: Claudia Kusmic, Area della Ricerca CNR, Istituto di Fisiologia Clinica, Via Moruzzi, 1-56124 Pisa, Italy. E-mail: kusmic{at}ifc.cnr.it

Accelerated cardiac washout of 123I-metaiodobenzylguanidine (MIBG), which is clinically used as an index of cardiac neuropathy in diabetes, is ascribed to decreased norepinephrine reuptake into synaptic vesicles. However, accelerated washout frequently contrasts with preserved early tracer uptake, whose significance remains undetermined. The aim of this study was to investigate in a mouse model of long-lasting type II diabetes whether the mismatch between MIBG early uptake and washout is the consequence of a more generalized disorder of the autonomic nervous system. Methods: Nine mice were given low doses of streptozotocin by intraperitoneal injection for 5 consecutive days. At 7 mo after streptozotocin, MIBG kinetics were evaluated by heart and liver time–activity curves and by tracer accumulation in the bladder. Data were compared with those obtained in 10 sham mice and correlated with the cardiac and hepatic tissue expression of norepinephrine transporter (NET) as assessed with a 3H-desipramine saturation binding assay. Results: In diabetic mice, myocardial and liver MIBG retention was reduced at 2 h and was associated with both increased tracer washout and reduced NET density. The rate of myocardial washout correlated with the degree of urinary MIBG excretion. Conclusion: The paradoxic observation of preserved early uptake associated with accelerated washout of MIBG in diabetes seems to be explained by a generalized disorder in NET function leading to reduced whole-body tracer removal from the blood and increased tracer availability for early myocardial uptake.

Key Words: type II diabetes • cardiac autonomic neuropathy • SPECT • radiobinding, mouse

COPYRIGHT © 2008 by the Society of Nuclear Medicine, Inc.


Related articles in JNM:

This Month in JNM

JNM 2008 49: 11A-12A. [Full Text]  





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS
JOURNAL OF NUCLEAR MEDICINE TECHNOLOGY THE JOURNAL OF NUCLEAR MEDICINE
Copyright © 2008 by the Society of Nuclear Medicine.