HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JNM Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tran, N. Articles by Marie, P.-Y. Search for Related Content PubMed PubMed Citation Articles by Tran, N. Articles by Marie, P.-Y.

Intramyocardial Implantation of Bone Marrow–Derived Stem Cells Enhances Perfusion in Chronic Myocardial Infarction: Dependency on Initial Perfusion Depth and Follow-up Assessed by Gated Pinhole SPECT

¹ School of Surgery, Faculty of Medicine-UHP, Nancy, France; ² UHP-INSERM U684, Nancy, France; ³ In Vivo Cellular and Molecular Imaging Center, University of Brussels, Brussels, Belgium; and ⁴ Department of Nuclear Medicine, CHU-Nancy, Nancy, France

Correspondence: For correspondence or reprints contact: Nguyen Tran, PhD, Laboratory of Surgery School, Faculty of Medicine, 9 Avenue de la Forêt de Haye, 54500 Vandoeuvre-lès-Nancy, France. E-mail: Nguyen.Tran{at}medecine.uhp-nancy.fr

Cell therapy–induced changes in the perfusion of areas of myocardial infarction (MI) remain unclear. This study investigated whether an original pinhole SPECT technique could be applied to a rat MI model to analyze local improvement in myocardial perfusion relating to engraftment sites of bone marrow–derived stem cells (BMSCs). Methods: Four-month-old MI rats were either untreated (n = 8) or treated (n = 10) by intramyocardial injection of ¹¹¹In-labeled BMSCs. Early distribution of ¹¹¹In-BMSCs within the MI target was evidenced by dual ¹¹¹In/^99mTc pinhole SPECT 48 h later. Myocardial perfusion was serially monitored by ^99mTc-sestamibi pinhole gated SPECT up to 3 mo after transplantation. Results: Forty-eight hours after transplantation, ¹¹¹In-BMSCs were observed in all treated rats and in 18 of their 32 underperfused MI segments (<70% sestamibi uptake before transplantation). During the subsequent 3-mo follow-up, the perfusion of MI segments worsened in untreated rats (absolute change in sestamibi uptake, –3% ± 3%; P < 0.05) but improved in treated rats (+4% ± 7%; P < 0.05). This perfusion improvement was unrelated to the initial detection of ¹¹¹In-BMSCs (+2% ± 6% in segments with ¹¹¹In-BMSCs vs. +5% ± 7% in those without; not statistically significant) but was strongly associated with less severe perfusion defects before transplantation (+6% ± 6% in segments with 60%–70% sestamibi uptake [n = 19] vs. –1% ± 6% in those with <60% uptake [n = 13]; P = 0.003). Conclusion: When BMSCs are injected within chronic MI, perfusion enhancement predominates in the MI areas showing a high enough residual perfusion before treatment but not in those of the initial cell engraftment, giving evidence of dependency on the perfusion and metabolic environment at implantation sites.

Key Words: myocardial infarction • rats • stem cell therapy • bone marrow mesenchymal stem cells • sestamibi • ¹¹¹In-oxine • pinhole SPECT

COPYRIGHT © 2007 by the Society of Nuclear Medicine, Inc.

Related articles in JNM:

This Month in JNM

JNM 2007 48: 9a-10a. [Full Text]  

This article has been cited by other articles:

				K. Kendziorra, H. Barthel, S. Erbs, F. Emmrich, R. Hambrecht, G. Schuler, O. Sabri, and R. Kluge Effect of Progenitor Cells on Myocardial Perfusion and Metabolism in Patients After Recanalization of a Chronically Occluded Coronary Artery J. Nucl. Med., April 1, 2008; 49(4): 557 - 563. [Abstract] [Full Text] [PDF]