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Multimodality Imaging of Tumor Xenografts and Metastases in Mice with Combined Small-Animal PET, Small-Animal CT, and Bioluminescence Imaging

¹ Molecular Imaging Program at Stanford (MIPS), Departments of Radiology and Bioengineering, Bio-X Program, Stanford University School of Medicine, Stanford University, Stanford, California; and ² Crump Institute for Molecular Imaging, Department of Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, California

Correspondence: For correspondence or reprints contact: Sanjiv S. Gambhir, MD, PhD, James H. Clark Center, 318 Campus Dr., East Wing, First Floor, Stanford, CA 94305-5427. E-mail: sgambhir{at}stanford.edu

Recent developments have established molecular imaging of mouse models with small-animal PET and bioluminescence imaging (BLI) as an important tool in cancer research. One of the disadvantages of these imaging modalities is the lack of anatomic information. We combined small-animal PET and BLI technology with small-animal CT to obtain fusion images with both molecular and anatomic information. Methods: We used small-animal PET/CT and BLI to detect xenografts of different cell lines and metastases of a melanoma cell line (A375M-3F) that had been transduced with a lentiviral vector containing a trimodality imaging reporter gene encoding a fusion protein with Renilla luciferase, monomeric red fluorescent protein, and a mutant herpes simplex virus type 1 thymidine kinase. Results: Validation studies in mouse xenograft models showed a good coregistration of images from both PET and CT. Melanoma metastases were detected by ¹⁸F-FDG PET, 9-[4-¹⁸F-fluoro-3-(hydroxymethyl)butyl]guanine (¹⁸F-FHBG) PET, CT, and BLI and confirmed by ex vivo assays of Renilla luciferase and mutant thymidine kinase expression. ¹⁸F-FHBG PET/CT allowed detection and localization of lesions that were not seen on CT because of poor contrast resolution and were not seen on ¹⁸F-FDG PET because of higher background uptake relative to ¹⁸F-FHBG. Conclusion: The combination of ¹⁸F-FHBG PET, small-animal CT, and BLI allows a sensitive and improved quantification of tumor burden in mice. This technique is potentially useful for the study of the biologic determinants of metastasis and for the evaluation of novel cancer treatments.

Key Words: microPET • small-animal CT • bioluminescence imaging • metastasis • mouse • melanoma

COPYRIGHT © 2007 by the Society of Nuclear Medicine, Inc.

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