JNM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JNM
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shichinohe, H.
Right arrow Articles by Iwasaki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shichinohe, H.
Right arrow Articles by Iwasaki, Y.
Journal of Nuclear Medicine Vol. 47 No. 3 486-491
© 2006 by Society of Nuclear Medicine

Basic Science Investigation

Improved Expression of {gamma}-Aminobutyric Acid Receptor in Mice with Cerebral Infarct and Transplanted Bone Marrow Stromal Cells: An Autoradiographic and Histologic Analysis

Hideo Shichinohe, MD, PhD1, Satoshi Kuroda, MD, PhD1, Shunsuke Yano, MD, PhD1, Takako Ohnishi, MSc2, Hiroshi Tamagami, MSc2, Kazutoshi Hida, MD, PhD1 and Yoshinobu Iwasaki, MD, PhD1

1 Department of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan; and 2 Research and Development Division, Research Center, Nihon Medi-Physics Co. Ltd., Sodegaura, Japan

Correspondence: For correspondence or reprints contact: Satoshi Kuroda, MD, PhD, Department of Neurosurgery, Hokkaido University Graduate School of Medicine, North 15 West 7, Kita-ku, Sapporo 060-8638, Japan. E-mail: skuroda{at}med.hokudai.ac.jp

Recent studies have indicated that bone marrow stromal cells (BMSC) have the potential to improve neurologic function when transplanted into animal models of central nervous system disorders. However, how the transplanted BMSC restore the lost neurologic function is not clear. In the present study, therefore, we aimed to elucidate whether BMSC express the neuron-specific {gamma}-aminobutyric acid (GABA) receptor when transplanted into brain that has been subjected to cerebral infarction. Methods: The BMSC were harvested from green fluorescent protein–transgenic mice and were cultured. The mice were subjected to permanent middle cerebral artery occlusion. The BMSC or vehicle was transplanted into the ipsilateral striatum 7 d after the insult. Using autoradiography and fluorescence immunohistochemistry, we evaluated the binding of 125I-iomazenil and the expression of GABA receptor protein in and around the cerebral infarct 4 wk after transplantation. Results: Binding of 125I-iomazenil was significantly higher in the periinfarct neocortex in the BMSC-transplanted animals than in the vehicle-transplanted animals. Likewise, the number of the GABAA receptor–positive cells was significantly higher in the periinfarct neocortex in the BMSC-transplanted animals than in the vehicle-transplanted animals. A certain subpopulation of the transplanted BMSC expressed a neuron-specific marker, microtubule-associated protein 2, and the marker protein specific for GABAA receptor in the periinfarct area. Conclusion: These findings suggest that BMSC may contribute to neural tissue regeneration through migrating toward the periinfarct area and acquiring the neuron-specific receptor function.

Key Words: bone marrow stromal cell • transplantation • cerebral infarct • iomazenil • autoradiography


Related articles in JNM:

This Month in JNM

JNM 2006 47: 7a-8a. [Full Text]  





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS
JOURNAL OF NUCLEAR MEDICINE TECHNOLOGY THE JOURNAL OF NUCLEAR MEDICINE
Copyright © 2006 by the Society of Nuclear Medicine.