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Accurate Noninvasive Measurement of Infarct Size in Mice with High-Resolution PET

¹ Department of Nuclear Medicine, University Hospital Münster, Münster, Germany; ² Interdisciplinary Center for Clinical Research, University Hospital Münster, Münster, Germany; ³ Institute of Anatomy, University Hospital Münster, Münster, Germany; and ⁴ Department of Anesthesiology and Intensive Care Medicine, University Hospital Münster, Münster, Germany

Correspondence: For correspondence or reprints contact: Lars Stegger, PhD, Department of Nuclear Medicine, University Hospital Münster, Albert-Schweitzer-Strasse 33, 48149, Münster, Germany. E-mail: stegger{at}uni-muenster.de

Reliable, repeatable, and time-efficient noninvasive measurement of infarct size in mice with PET would benefit studies aimed at the exploration of biochemical and functional changes associated with acute myocardial infarction (MI). PET with the radioactively labeled glucose derivative ¹⁸F-FDG is used in humans to distinguish between viable but dysfunctional and nonviable myocardium. In this study, the feasibility, accuracy, and time efficiency of ¹⁸F-FDG PET for quantification of infarct size in mice using a high-resolution animal PET device was evaluated in comparison with histomorphometry. Methods: Mice were subjected to surgery with permanent ligation of the left anterior descending artery. PET was performed before and 7 d after surgery. The infarct size was determined from the PET studies using both manual and automated delineation. The second PET scan was followed by histomorphometric analysis. Results: An excellent correlation between PET and histomorphometry was found for both manual (R = 0.98) and automated (R = 0.98) delineation, with linear regression curves close to unity (manual: y = 1.10x – 0.01; automated: y = 1.12x – 0.02). Automated analysis required <1 min per study. Conclusion: The measurement of infarct size in mice with ¹⁸F-FDG PET is feasible and highly accurate. This noninvasive methodology permits unique longitudinal studies of biochemical parameters in mice and facilitates studies that aim to assess the effect of surgical and pharmacologic intervention after acute MI.

Key Words: infarct size • quantification • mouse • rodent • PET • ¹⁸F-FDG

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