HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rimoldi, O. Articles by Camici, P. G. Search for Related Content PubMed PubMed Citation Articles by Rimoldi, O. Articles by Camici, P. G.

Quantification of Subendocardial and Subepicardial Blood Flow Using ¹⁵O-Labeled Water and PET: Experimental Validation

¹ Medical Research Council Clinical Sciences Centre, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Hammersmith Hospital, London, United Kingdom; ² Department of Nuclear Medicine, University of Münster, Münster, Germany; ³ Department of Nuclear Medicine and PET Research, VU University Medical Center, Amsterdam, The Netherlands; and ⁴ Sensomotor Department, Imperial College London, United Kingdom

Correspondence: For correspondence or reprints contact: Ornella Rimoldi, MD, MRC Clinical Sciences Centre, Hammersmith Hospital, Du Cane Rd., London W12 ONN, U.K. E-mail: ornella.rimoldi{at}csc.mrc.ac.uk

The purpose of this study was to assess the feasibility and accuracy of quantifying subendocardial and subepicardial myocardial blood flow (MBF) and the relative coronary flow reserves (CFR) using ¹⁵O-labeled water (H₂¹⁵O) and 3-dimensional–only PET. Methods: Eight pigs were scanned with H₂¹⁵O and ¹⁵O-labeled carbon monoxide (C¹⁵O) after partially occluding the circumflex (n = 3) or the left anterior descending (n = 5) coronary artery, both at rest and during hyperemia induced by intravenous dipyridamole. Radioactive microspheres were injected during each of the H₂¹⁵O scans. Results: In a total of 256 paired measurements of MBF, ranging from 0.30 to 4.46 mL·g^–1·min^–1, microsphere and PET MBF were fairly well correlated. The mean difference between the 2 methods was –0.01 ± 0.52 mL·g^–1·min^–1 with 95% of the differences lying between the limits of agreement of –1.02 and 1.01 mL·g^–1·min^–1. CFR was significantly reduced (P < 0.05) in the ischemic subendocardium (PET = 1.12 ± 0.45; microspheres = 1.09 ± 0.50; P = 0.86) and subepicardium (PET = 1.2 ± 0.35; microspheres = 1.32 ± 0.5; P = 0.39) in comparison with remote subendocardium (PET = 1.7 ± 0.62; microspheres = 1.64 ± 0.61; P = 0.68) and subepicardium (PET = 1.79 ± 0.73; microspheres = 2.19 ± 0.86; P = 0.06). Conclusion: Dynamic measurements using H₂¹⁵O and a 3-dimensional–only PET tomograph allow regional estimates of the transmural distribution of MBF over a wide flow range, although transmural flow differences were underestimated because of the partial-volume effect. PET subendocardial and subepicardial CFR were in good agreement with the microsphere values.

Key Words: myocardial blood flow • 3-dimensional PET • H₂¹⁵O • subendocardial ischemia

This article has been cited by other articles:

				A. J. de Langen, V. E. M. van den Boogaart, J. T. Marcus, and M. Lubberink Use of H215O-PET and DCE-MRI to Measure Tumor Blood Flow Oncologist, June 1, 2008; 13(6): 631 - 644. [Abstract] [Full Text] [PDF]

				P. Knaapen, T. Germans, P. G. Camici, O. E. Rimoldi, F. J. ten Cate, J. M. ten Berg, P. A. Dijkmans, R. Boellaard, W. G. van Dockum, M. J. W. Gotte, et al. Determinants of coronary microvascular dysfunction in symptomatic hypertrophic cardiomyopathy Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H986 - H993. [Abstract] [Full Text] [PDF]