HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JNM Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Constantinesco, A. Articles by Mertz, L. Search for Related Content PubMed PubMed Citation Articles by Constantinesco, A. Articles by Mertz, L.

Assessment of Left Ventricular Perfusion, Volumes, and Motion in Mice Using Pinhole Gated SPECT

¹ Service de Biophysique et Médecine Nucléaire, Hôpital de Hautepierre, Strasbourg, France
² Institut Clinique de la Souris, Illkirch, France
³ Institut de Recherche Mathématique Avancée, Strasbourg, France

Quantitative functional normal data should be a prerequisite before applying SPECT in murine models of cardiac disease. Therefore, we investigated the capability of in vivo pinhole gated SPECT for establishment of a reference database for left ventricular myocardial perfusion, volumes, and motion in normal mice. Methods: A small-animal dedicated pinhole -camera with a field of view of 17 cm and a focal distance of 12 cm was used with a 1.5-mm pinhole and a 2.5-cm radius of rotation. Phantoms were designed to test spatial resolution and microvolume measurements of accuracy. Eight adult normal mice (CD1) were studied using a heated mixture of air (0.3 L/min) and 1.5%–2.5% isoflurane for anesthesia. For myocardial perfusion, 350–450 MBq of ^99mTc-tetrofosmin were used in 0.15–0.25 mL. Gated acquisitions (8 or 10 time bins per cardiac cycle) were obtained using a 180° circular arc and 48 anterior projections of 300 R–R intervals. Image reconstruction was done using a specific Algebraic Reconstruction Technique (ART) cone-beam algorithm. For quantification, reconstructed images were processed using standard nuclear medicine software. Results: Millimetric spatial resolution and volume calibration linear relationships (r² = 0.99) in the 10- to 100-µL range were obtained in phantoms and used to scale in vivo volume values. In mice, left ventricular perfusion was lower in the apex (65% ± 6%) versus lateral (72% ± 5%), inferior (74% ± 5%), septum (75% ± 4%), and anterior (74% ± 2%) walls. The left ventricular ejection fraction was 60% ± 9%, end-diastolic volume was 50 ± 8 µL, end-systolic volume was 20 ± 6 µL, stroke volume was 29.5 ± 6 µL, and cardiac output was 9.6 ± 1.6 mL/min. Wall thickening was higher in the apex (47% ± 12%) versus lateral (30% ± 9%), inferior (33% ± 8%), septum (37% ± 10%), and anterior (33% ± 10%) walls. Conclusion: This work shows that in vivo pinhole gated SPECT can be used for assessment of left ventricular perfusion, volumes, and cardiac function in normal mice.

Key Words: mice • gated SPECT • myocardial perfusion • ventricular function • molecular imaging

Related articles in JNM:

THIS MONTH IN JNM

JNM 2005 46: 11a-12a. [Full Text]  

This article has been cited by other articles:

				B. M.W. Tsui and D. L. Kraitchman Recent Advances in Small-Animal Cardiovascular Imaging J. Nucl. Med., May 1, 2009; 50(5): 667 - 670. [Abstract] [Full Text] [PDF]

				L. Stegger, E. Heijman, K. P. Schafers, K. Nicolay, M. A. Schafers, and G. J. Strijkers Quantification of Left Ventricular Volumes and Ejection Fraction in Mice Using PET, Compared with MRI J. Nucl. Med., January 1, 2009; 50(1): 132 - 138. [Abstract] [Full Text] [PDF]

				C. Goetz, E. Breton, P. Choquet, V. Israel-Jost, and A. Constantinesco SPECT Low-Field MRI System for Small-Animal Imaging J. Nucl. Med., January 1, 2008; 49(1): 88 - 93. [Abstract] [Full Text] [PDF]