HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JNM Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, D. L. Articles by Schuster, D. P. Search for Related Content PubMed PubMed Citation Articles by Chen, D. L. Articles by Schuster, D. P.

Comparison of Methods to Quantitate ¹⁸F-FDG Uptake with PET During Experimental Acute Lung Injury

¹ Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
² Department of Surgery, Washington University School of Medicine, St. Louis, Missouri
³ Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri

PET with ¹⁸F-FDG may be useful for quantifying neutrophilic activation. We previously demonstrated that pulmonary neutrophil sequestration could be detected during acute lung injury (ALI), even without migration into the alveolar compartment. Using the influx constant K_i as the method to quantify lung ¹⁸F-FDG uptake, we also showed that K_i correlated positively with in vitro assays of ³H-deoxyglucose (³H-DG) uptake in cells harvested via bronchoalveolar lavage. In the present study, we have reanalyzed data from that study to determine if simpler nonkinetic methods of quantifying the pulmonary uptake of ¹⁸F-FDG could be as powerful as calculating K_i. Methods: ¹⁸F-FDG uptake was quantified as K_i, calculated by 3-compartmental model analysis (used as the gold standard) and Patlak graphical analysis, with and without normalization for initial volume of tracer distribution; the standardized uptake value; and the tissue-to-plasma activity ratio (TPR). Results: Values for K_i, determined either from a 3-compartmental model analysis of the time–activity data or by Patlak graphical analysis, were highly correlated (R² = 0.97). The correlation was worse if these variables were normalized for the initial volume of tracer distribution. TPR was highly correlated with K_i determined by the compartmental model (R² = 0.96) and with in vitro measurements of ³H-DG uptake (R² = 0.63). Conclusion: The TPR is a simple and equally effective alternative to dynamic imaging in determining net ¹⁸F-FDG uptake during ALI. Normalization of the kinetic data for differences in the initial volume of tracer distribution does not contribute significantly to signal interpretation during ALI.

Key Words: FDG • compartmental model • acute lung injury • inflammation imaging • PET

Related articles in JNM:

THIS MONTH IN JNM

JNM 2004 45: 8A-9A. [Full Text]  

This article has been cited by other articles:

				T. Schroeder, M. F. Vidal Melo, G. Musch, R. S. Harris, J. G. Venegas, and T. Winkler Image-Derived Input Function for Assessment of 18F-FDG Uptake by the Inflamed Lung J. Nucl. Med., November 1, 2007; 48(11): 1889 - 1896. [Abstract] [Full Text] [PDF]

				M. B. Dolovich and D. P. Schuster Positron Emission Tomography and Computed Tomography versus Positron Emission Tomography Computed Tomography: Tools for Imaging the Lung Proceedings of the ATS, August 1, 2007; 4(4): 328 - 333. [Abstract] [Full Text] [PDF]

				D. P. Schuster, S. L. Brody, Z. Zhou, M. Bernstein, R. Arch, D. Link, and M. Mueckler Regulation of lipopolysaccharide-induced increases in neutrophil glucose uptake Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L845 - L851. [Abstract] [Full Text] [PDF]

				T. Schroeder, M. F. Vidal Melo, G. Musch, R. S. Harris, T. Winkler, and J. G. Venegas PET Imaging of Regional 18F-FDG Uptake and Lung Function After Cigarette Smoke Inhalation J. Nucl. Med., March 1, 2007; 48(3): 413 - 419. [Abstract] [Full Text] [PDF]

				D. L. Chen, T. W. Ferkol, M. A. Mintun, J. E. Pittman, D. B. Rosenbluth, and D. P. Schuster Quantifying Pulmonary Inflammation in Cystic Fibrosis with Positron Emission Tomography Am. J. Respir. Crit. Care Med., June 15, 2006; 173(12): 1363 - 1369. [Abstract] [Full Text] [PDF]

				D. L. Chen, D. B. Rosenbluth, M. A. Mintun, and D. P. Schuster FDG-PET imaging of pulmonary inflammation in healthy volunteers after airway instillation of endotoxin J Appl Physiol, May 1, 2006; 100(5): 1602 - 1609. [Abstract] [Full Text] [PDF]

				Z. Zhou, J. Kozlowski, A. L. Goodrich, N. Markman, D. L. Chen, and D. P. Schuster Molecular imaging of lung glucose uptake after endotoxin in mice Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L760 - L768. [Abstract] [Full Text] [PDF]