HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JNM Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Taki, J. Articles by Strauss, H. W. Search for Related Content PubMed PubMed Citation Articles by Taki, J. Articles by Strauss, H. W.

Detection of Cardiomyocyte Death in a Rat Model of Ischemia and Reperfusion Using ^99mTc-Labeled Annexin V

¹ Department of Biotracer Medicine, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Japan
² Department of Molecular and Cellular Pathology, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Japan
³ Department of Laboratory Medicine, University of Washington, Seattle, Washington
⁴ Medical and Pharmacological Research Center Foundation, Hakui, Japan
⁵ Division of Nuclear Medicine, Department of Radiology, Memorial Sloan-Kettering Hospital, New York, New York

There is increasing evidence that cell death after myocardial ischemia and reperfusion may begin as apoptosis rather than necrosis. To determine the time course, location, and extent of this process, we studied groups of rats after a 20-min interval of coronary occlusion and reperfusion. Methods: After thoracotomy, the left coronary artery was occluded for 20 min. After release and before study, groups of animals were allowed to recover for various intervals: 0.5 h (n = 6), 1.5 h (n = 7), 6 h (n = 7), 1 d (n = 8), 3 d (n = 8), or 2 wk (n = 5). At the time of study, the rats were injected with ^99mTc-annexin V (80–150 MBq). One hour later, to verify the area at risk, ²⁰¹Tl (0.74 MBq) was injected intravenously just after the left coronary artery reocclusion and the rats were sacrificed 1 min later. Dual-tracer autoradiography was performed to assess ^99mTc-annexin V uptake and the area at risk. Results: Extensive ^99mTc-annexin V uptake was observed in the mid myocardium after 0.5–1.5 h of reperfusion. The area of annexin uptake had expanded in the subendocardial and subepicardial layers at 6 h after reperfusion and then gradually lessened over 3 d. At 0.5 and 1.5 h of reperfusion, ^99mTc-annexin V uptake ratios were 7.36 ± 2.95 and 6.34 ± 2.24 (mean ± SD), respectively. The uptake ratios gradually decreased at 6 h, 1 d, 3 d, and 2 wk after reperfusion (4.65 ± 1.93, 3.27 ± 0.92 [P < 0.01 vs. 0.5 h], 1.84 ± 0.55 [P < 0.001 vs. 0.5 h, P < 0.005 vs. 1.5 h], and 1.65 ± 0.31 [P < 0.001 vs. 0.5 h, P < 0.005 vs. 1.5 h], respectively). Conclusion: These data indicate that annexin binding commences soon after ischemia and reperfusion in the mid myocardium within the area at risk and expands to include the subendocardial and subepicardial layers at 6 h after reperfusion, followed by gradual reduction of activity over 3 d.

Key Words: ^99mTc-annexin V • ischemia • reperfusion • apoptosis

Related articles in JNM:

THIS MONTH IN JNM

JNM 2004 45: 8A-9A. [Full Text]  

This article has been cited by other articles:

				T. Doue, K. Ohtsuki, K. Ogawa, M. Ueda, A. Azuma, H. Saji, H. W. Strauss, and H. Matsubara Cardioprotective Effects of Erythropoietin in Rats Subjected to Ischemia-Reperfusion Injury: Assessment of Infarct Size with 99mTc-Annexin V J. Nucl. Med., October 1, 2008; 49(10): 1694 - 1700. [Abstract] [Full Text] [PDF]

				T. Higuchi, F. M. Bengel, S. Seidl, P. Watzlowik, H. Kessler, R. Hegenloh, S. Reder, S. G. Nekolla, H. J. Wester, and M. Schwaiger Assessment of {alpha}v{beta}3 integrin expression after myocardial infarction by positron emission tomography Cardiovasc Res, May 1, 2008; 78(2): 395 - 403. [Abstract] [Full Text] [PDF]

				J. Taki, T. Higuchi, A. Kawashima, M. Fukuoka, D. Kayano, J. F. Tait, I. Matsunari, K. Nakajima, S. Kinuya, and H. W. Strauss Effect of Postconditioning on Myocardial 99mTc-Annexin-V Uptake: Comparison with Ischemic Preconditioning and Caspase Inhibitor Treatment J. Nucl. Med., August 1, 2007; 48(8): 1301 - 1307. [Abstract] [Full Text] [PDF]

				T. Higuchi, S. G. Nekolla, A. Jankaukas, A. W. Weber, M. C. Huisman, S. Reder, S. I. Ziegler, M. Schwaiger, and F. M. Bengel Characterization of Normal and Infarcted Rat Myocardium Using a Combination of Small-Animal PET and Clinical MRI J. Nucl. Med., February 1, 2007; 48(2): 288 - 294. [Abstract] [Full Text] [PDF]

				M. Zhao, X. Zhu, S. Ji, J. Zhou, K. S. Ozker, W. Fang, R. C. Molthen, and R. S. Hellman 99mTc-Labeled C2A Domain of Synaptotagmin I as a Target-Specific Molecular Probe for Noninvasive Imaging of Acute Myocardial Infarction J. Nucl. Med., August 1, 2006; 47(8): 1367 - 1374. [Abstract] [Full Text] [PDF]

				H. H. Boersma, B. L.J.H. Kietselaer, L. M.L. Stolk, A. Bennaghmouch, L. Hofstra, J. Narula, G. A.K. Heidendal, and C. P.M. Reutelingsperger Past, Present, and Future of Annexin A5: From Protein Discovery to Clinical Applications J. Nucl. Med., December 1, 2005; 46(12): 2035 - 2050. [Abstract] [Full Text] [PDF]

				N S Kleiman and H D White The declining prevalence of ST elevation myocardial infarction in patients presenting with acute coronary syndromes Heart, September 1, 2005; 91(9): 1121 - 1123. [Abstract] [Full Text] [PDF]