HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Peker, C. Articles by Le Guludec, D. Search for Related Content PubMed PubMed Citation Articles by Peker, C. Articles by Le Guludec, D.

Imaging Apoptosis with ^99mTc-Annexin-V in Experimental Subacute Myocarditis

¹ Équipe d’Accueil 35 12, Faculté Bichat-Claude Bernard et Service de Médecine Nucléaire de l’hôpital Bichat, Assistance Publique-Hôpitaux de Paris, Paris, France
² Institut National de la Santé et de la Recherche Médicale, Unité 460, Unité de Formation et de Recherche Bichat, Paris, France

^99mTc-Annexin-V (ANX), which allows in vivo detection of apoptotic cells, is potentially a promising noninvasive tool to diagnose myocarditis. To test this assumption, we compared the myocardial uptake of ANX (imaging and quantitative autoradiography) in experimental subacute myocarditis (Wistar Bonn/Kobori rats [WBN/Kob]) and in normal Wistar rats. WBN/Kob is an inbred strain of Wistar rat in which myocardial injury mimicking subacute catecholamine-induced myocarditis spontaneously develops (course duration, 18 mo). The apoptotic myocardial rates were determined by immunohistochemical studies. Methods: Fourteen WBN/Kob rats (8–10 mo old) and 12 control rats were injected with ANX (7.4 MBq/100 g). Ten-minute anterior planar thoracic images (matrix, 128 x 128) were obtained using a pinhole collimator, 1 and 4 h after injection. Heart-to-lung activity ratios were calculated on the scintigrams. Four hours after ANX injection, quantitative autoradiography of myocardial slices was performed, as well as histologic studies with hematoxylin–eosin and with a staining assay specific for apoptotic cells. Results: Heart-to-lung activity ratios were higher in WBN/Kob rats than in control rats on 4-h images (2.07 ± 0.07 vs. 1.66 ± 0.06, P = 0.0007). Autoradiographic studies showed moderate diffuse, homogeneous myocardial ANX uptake significantly higher in WBN/Kob rats than in control rats: 54 ± 4 versus 37 ± 3 counts/mm² (P < 0.007). The apoptotic rate, evaluated with an apoptotic cell–staining assay, was 0.51% ± 0.14% of cells in WBN/Kob rats versus 0.0042% ± 0.0008% in control rats (P < 0.008). Conclusion: Compared with control rats, rats with subacute myocarditis mimicking catecholamine-induced myocarditis showed increased ANX myocardial uptake. This suggests a potential role for ANX imaging in the diagnosis of myocarditis.

Key Words: apoptosis • ^99mTc-annexin-V • myocarditis

This article has been cited by other articles:

				A. Saraste, S. G. Nekolla, and M. Schwaiger Cardiovascular molecular imaging: an overview Cardiovasc Res, September 1, 2009; 83(4): 643 - 652. [Abstract] [Full Text] [PDF]

				F. G. Blankenberg Imaging the Molecular Signatures of Apoptosis and Injury with Radiolabeled Annexin V Proceedings of the ATS, August 15, 2009; 6(5): 469 - 476. [Abstract] [Full Text] [PDF]

				T. Doue, K. Ohtsuki, K. Ogawa, M. Ueda, A. Azuma, H. Saji, H. W. Strauss, and H. Matsubara Cardioprotective Effects of Erythropoietin in Rats Subjected to Ischemia-Reperfusion Injury: Assessment of Infarct Size with 99mTc-Annexin V J. Nucl. Med., October 1, 2008; 49(10): 1694 - 1700. [Abstract] [Full Text] [PDF]

				F. Rouzet, M. D. Hernandez, F. Hervatin, L. Sarda-Mantel, A. Lefort, X. Duval, L. Louedec, B. Fantin, D. Le Guludec, and J.-B. Michel Technetium 99m-Labeled Annexin V Scintigraphy of Platelet Activation in Vegetations of Experimental Endocarditis Circulation, February 12, 2008; 117(6): 781 - 789. [Abstract] [Full Text] [PDF]

				H. N. Skouri, G. W. Dec, M. G. Friedrich, and L. T. Cooper Noninvasive Imaging in Myocarditis J. Am. Coll. Cardiol., November 21, 2006; 48(10): 2085 - 2093. [Abstract] [Full Text] [PDF]

				L. Sarda-Mantel, M. Coutard, F. Rouzet, O. Raguin, J.-M. Vrigneaud, F. Hervatin, G. Martet, Z. Touat, P. Merlet, D. Le Guludec, et al. 99mTc-Annexin-V Functional Imaging of Luminal Thrombus Activity in Abdominal Aortic Aneurysms Arterioscler Thromb Vasc Biol, September 1, 2006; 26(9): 2153 - 2159. [Abstract] [Full Text] [PDF]