HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hattori, N. Articles by Huang, S.-C. Search for Related Content PubMed PubMed Citation Articles by Hattori, N. Articles by Huang, S.-C.

Accuracy of a Method Using Short Inhalation of ¹⁵O-O₂ for Measuring Cerebral Oxygen Extraction Fraction with PET in Healthy Humans

¹ Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California
² Brain Injury Research Center, UCLA Division of Neurosurgery, David Geffen School of Medicine at UCLA, Los Angeles, California
³ UCLA-DOE Center for Molecular Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California

PET with short inhalation of ¹⁵O-O₂ provides regional oxygen extraction fraction (OEF) in a shorter acquisition time and with less radiation exposure than does the steady-state method. The purpose of this study was to test the accuracy of the short-inhalation technique for estimating OEF in healthy human volunteers. Methods: The final study population included 16 healthy volunteers, who underwent a series of dynamic PET scans consisting of short inhalation of ¹⁵O-CO, short inhalation of ¹⁵O-O₂, and a bolus infusion of ¹⁵O-H₂O to generate parametric images for cerebral blood volume (CBV), cerebral blood flow (CBF), OEF, and metabolic rate of oxygen (CMRO₂). About 45 min before PET emission scanning, arterial and jugular blood was sampled through a catheter inserted in a radial artery and the right jugular bulb, respectively. PET-derived OEF (OEFpet) of the whole brain was compared with OEF calculated from the arteriovenous blood-sampling technique (OEFav). Results: Whole-brain-averaged CBF (mean ± SD) measured with PET was 0.40 ± 0.06 (range, 0.30–0.55) mL/g/min, CBV was 0.05 ± 0.01 (range, 0.04–0.09) mL/g, CMRO₂ was 2.85 ± 0.39 (range, 2.35–3.84) mL/100 g/min, and OEFpet was 0.39 ± 0.06 (range, 0.30–0.51). OEFpet showed a slightly higher value than did OEFav (0.36 ± 0.05 [range, 0.29–0.46]), but the difference was not significant. The difference in the 2 measurements (OEFpet - OEFav) did not correlate with CBF (r = –0.16; P = not statistically significant [NS]), CBV (r = –0.20; P = NS), CMRO₂ (r = –0.16; P = NS), partial arterial oxygen pressure (r = 0.29; P = NS) or partial arterial carbon dioxide pressure (r = –0.17; P = NS). Conclusion: Compared with the arteriovenous blood-sampling technique, a technique using short inhalation of ¹⁵O-O₂ did not significantly over- or underestimate global OEF in healthy human volunteers. The PET technique reasonably estimated the cerebral OEF in local brain tissues of healthy human volunteers.

Key Words: PET • arteriovenous oxygen difference • oxygen extraction fraction • short inhalation of oxygen gas • validation

This article has been cited by other articles:

				H. An, Q. Liu, Y. Chen, and W. Lin Evaluation of MR-Derived Cerebral Oxygen Metabolic Index in Experimental Hyperoxic Hypercapnia, Hypoxia, and Ischemia Stroke, June 1, 2009; 40(6): 2165 - 2172. [Abstract] [Full Text] [PDF]