HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Niu, G. Articles by Domann, F. E. Search for Related Content PubMed PubMed Citation Articles by Niu, G. Articles by Domann, F. E.

Multimodality Noninvasive Imaging of Gene Transfer Using the Human Sodium Iodide Symporter

¹ Free Radical and Radiation Biology Program, Department of Radiation Oncology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa
² Department of Otolaryngology-Head and Neck Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa
³ Division of Nuclear Medicine, Department of Radiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa

ABSTRACT

In this study we investigated the feasibility of using radionuclide accumulation mediated by the human sodium iodide symporter (hNIS) gene in conjunction with various imaging modalities as a reporter system to noninvasively monitor the expression of transgenes delivered for gene therapy. Methods: NIS-expressing adenovirus (Ad-hNIS) was delivered in vitro to MB-435 breast carcinoma cells. NIS-mediated accumulation of ¹²⁵I^-, ^99mTcO₄^-, and ⁷⁶Br^- by the cells was visualized using autoradiography, -camera scintigraphy, and PET imaging, respectively. Results: For all imaging modalities, signal intensity generated by the cells correlated linearly both with the amount of Ad-hNIS and with the activity of radionuclide added to the cells. Conclusion: hNIS-mediated cellular accumulation of radionuclide was clearly visualized by all 3 imaging modalities tested. This preliminary study demonstrates the feasibility of using hNIS for monitoring the location and magnitude of expression of genes delivered during gene therapy.

Key Words: sodium iodide symporter • gene therapy • gene transfer • reporter gene • molecular imaging

This article has been cited by other articles:

				Z. Lee, J. E. Dennis, and S. L. Gerson Imaging Stem Cell Implant for Cellular-Based Therapies Experimental Biology and Medicine, August 1, 2008; 233(8): 930 - 940. [Abstract] [Full Text] [PDF]

				R. Zhou, P. D. Acton, and V. A. Ferrari Imaging Stem Cells Implanted in Infarcted Myocardium J. Am. Coll. Cardiol., November 21, 2006; 48(10): 2094 - 2106. [Abstract] [Full Text] [PDF]

				Z. Zhang, Y.-Y. Liu, and S. M. Jhiang Cell Surface Targeting Accounts for the Difference in Iodide Uptake Activity between Human Na+/I- Symporter and Rat Na+/I- Symporter J. Clin. Endocrinol. Metab., November 1, 2005; 90(11): 6131 - 6140. [Abstract] [Full Text] [PDF]

				D. J. Buchsbaum, T. R. Chaudhuri, and K. R. Zinn Radiotargeted Gene Therapy J. Nucl. Med., January 1, 2005; 46(1_suppl): 179S - 186S. [Abstract] [Full Text] [PDF]

				M. Miyagawa, M. Beyer, B. Wagner, M. Anton, C. Spitzweg, B. Gansbacher, M. Schwaiger, and F. M. Bengel Cardiac reporter gene imaging using the human sodium/iodide symporter gene Cardiovasc Res, January 1, 2005; 65(1): 195 - 202. [Abstract] [Full Text] [PDF]