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Simple Analytic Method of ¹¹C-Flumazenil Metabolite in Blood

¹ Photon Medical Research Center, Hamamatsu University School of Medicine, Hamamatsu, Japan
² Department of Nuclear Medicine and Diagnostic Imaging, Graduate School of Medicine, Kyoto University, Kyoto, Japan
³ Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
⁴ Division of Medical Imaging, Biomedical Imaging Research Center, Fukui Medical University, Fukui, Japan
⁵ Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan

ABSTRACT

¹¹C-Flumazenil (¹¹C-FMZ) is useful to estimate central benzodiazepine receptors by PET. The binding potential (BP) can be calculated with dynamic PET and continual blood sampling. Because conventional metabolite analysis of plasma samples is complicated and time consuming, a simple method is required to obtain an input function. In this article, a whole blood solvent extraction method was evaluated using data of 13 subjects. Methods: The plasma solvent extraction method was estimated in comparison with the thin-layer chromatography (TLC) method. Then, the whole blood solvent extraction method was evaluated in comparison with the plasma solvent extraction method. Results: Metabolite data analyzed by the plasma extraction method were well correlated with those by the TLC method (r = 0.99). The BP was calculated using both the whole blood extraction data and the plasma extraction data. No difference was observed in all brain regions. Conclusion: This simple whole blood solvent extraction method can be applied to clinical BP estimation using ¹¹C-FMZ.

Key Words: positron • benzodiazemine receptor • flumazenil • input function

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