HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ishimori, T. Articles by Konishi, J. Search for Related Content PubMed PubMed Citation Articles by Ishimori, T. Articles by Konishi, J.

Increased ¹⁸F-FDG Uptake in a Model of Inflammation: Concanavalin A-Mediated Lymphocyte Activation

¹ Department of Nuclear Medicine and Diagnostic Imaging, Graduate School of Medicine, Kyoto University, Kyoto, Japan
² Department of Diagnostic and Interventional Imagiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan

The aim of this project was to study a mechanism that might explain the increased uptake of ¹⁸F-labeled FDG seen in inflammation. The approach chosen was to examine the effect on ¹⁸F-FDG uptake of acute activation of murine lymphocytes by concanavalin A (Con A). Methods: Immunocompetent BALB/c mice and nude mice received an intravenous injection of 10 mg/kg Con A. Twenty-four hours later, the mice received an intravenous injection of 0.74 MBq (20 µCi) ¹⁸F-FDG. One hour later, biodistribution was determined. The distribution of the radiolabel in the liver was also evaluated by autoradiography. In vitro ¹⁸F-FDG uptake to splenocytes from BALB/c mice with and without Con A pretreatment were determined 30, 60, and 120 min after the splenocytes were mixed with ¹⁸F-FDG (0.74 MBq [20 µCi]/200 µL). Results: In immunocompetent BALB/c mice, pretreatment with Con A significantly increased ¹⁸F-FDG uptake in the spleen and liver. Autoradiographs of the liver showed that pretreatment with Con A produced a specific localization of ¹⁸F-FDG at periportal areas, where numerous sites of cellular infiltration were observed. In vitro binding of ¹⁸F-FDG to the splenocytes was significantly higher for Con A-pretreated BALB/c mice than for control mice. Conclusion: This study showed that Con A increased ¹⁸F-FDG uptake. Con A-activated lymphocytes actively took up ¹⁸F-FDG both in vitro and in vivo, and ¹⁸F-FDG specifically accumulated in Con A-mediated acute inflammatory tissues.

Key Words: ¹⁸F-FDG PET • concanavalin A • lymphocyte • acute inflammation

This article has been cited by other articles:

				T. Kalliokoski, P. Nuutila, K. A. Virtanen, P. Iozzo, M. Bucci, E. Svedstrom, A. Roivainen, K. Nagren, T. Viljanen, H. Minn, et al. Pancreatic Glucose Uptake in Vivo in Men with Newly Diagnosed Type 1 Diabetes J. Clin. Endocrinol. Metab., May 1, 2008; 93(5): 1909 - 1914. [Abstract] [Full Text] [PDF]

				N. Tahara, H. Kai, M. Ishibashi, H. Nakaura, H. Kaida, K. Baba, N. Hayabuchi, and T. Imaizumi Simvastatin Attenuates Plaque Inflammation: Evaluation by Fluorodeoxyglucose Positron Emission Tomography J. Am. Coll. Cardiol., November 7, 2006; 48(9): 1825 - 1831. [Abstract] [Full Text] [PDF]

				S. KAWAI, E. IKEDA, M. SUGIYAMA, J. MATSUMOTO, T. HIGUCHI, H. ZHANG, N. KHAN, K. TOMIYOSHI, T. INOUE, H. YAMAGUCHI, et al. ENHANCEMENT OF SPLENIC GLUCOSE METABOLISM DURING ACUTE MALARIAL INFECTION: CORRELATION OF FINDINGS OF FDG-PET IMAGING WITH PATHOLOGICAL CHANGES IN A PRIMATE MODEL OF SEVERE HUMAN MALARIA. Am J Trop Med Hyg, March 1, 2006; 74(3): 353 - 360. [Abstract] [Full Text] [PDF]

				H. L. van Westreenen, D. C.P. Cobben, P. L. Jager, H. M. van Dullemen, J. Wesseling, P. H. Elsinga, and J. Th. Plukker Comparison of 18F-FLT PET and 18F-FDG PET in Esophageal Cancer J. Nucl. Med., March 1, 2005; 46(3): 400 - 404. [Abstract] [Full Text] [PDF]

				J.-J. Min, S. Biswal, C. Deroose, and S. S. Gambhir Tetraphenylphosphonium as a Novel Molecular Probe for Imaging Tumors J. Nucl. Med., April 1, 2004; 45(4): 636 - 643. [Abstract] [Full Text] [PDF]

				S. Maschauer, O. Prante, M. Hoffmann, J. T. Deichen, and T. Kuwert Characterization of 18F-FDG Uptake in Human Endothelial Cells In Vitro J. Nucl. Med., March 1, 2004; 45(3): 455 - 460. [Abstract] [Full Text]

				H. L. van Westreenen, P. A.M. Heeren, P. L. Jager, H. M. van Dullemen, H. Groen, and J. Th.M. Plukker Pitfalls of Positive Findings in Staging Esophageal Cancer With F-18-Fluorodeoxyglucose Positron Emission Tomography Ann. Surg. Oncol., November 1, 2003; 10(9): 1100 - 1105. [Abstract] [Full Text] [PDF]