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Chemotaxis, Viability, and Labeling Stability of Leukocytes Labeled with ^99mTc-Exametazime Stabilized with Methylene Blue

Radiopharmacy Unit, Nuclear Medicine Department, L’Hospitalet de Llobregat, Ciutat Sanitària Universitària de Bellvitge, Barcelona, Spain

The in vitro viability, chemotaxis, and labeling stability of leukocytes labeled using ^99mTc-exametazime stabilized with methylene blue were evaluated and compared with those obtained using nonstabilized ^99mTc-exametazime. Methods: Two identical leukocyte populations, from 30 healthy donors, were labeled simultaneously using freshly prepared and 2-h-old stabilized ^99mTc-exametazime. The following quality control techniques were performed on each labeled leukocyte sample: eosin Y staining, chemotaxis radioassay, and labeling stability at 2 h after labeling. Results: Eosin Y staining showed a cell viability of at least 98% in all samples, without a significant statistical difference between the populations. Chemotactic indices obtained with leukocytes labeled with freshly prepared, unstabilized ^99mTc-exametazime were statistically greater than those obtained using ^99mTc-exametazime stabilized with methylene blue (z = 2.41; P < 0.02). Labeling stability at 2 h after labeling was the same for both populations. Conclusion: The use of ^99mTc-exametazime stabilized with methylene blue for leukocyte radiolabeling does not affect either cell membrane integrity or labeling stability but can cause a decrease in the cell chemotactic capacity that discourages its clinical use.

Key Words: leukocytes • ^99mTc-exametazime • radiopharmaceutical stabilization • methylene blue • chemotaxis

This article has been cited by other articles:

				J. C. Hung, B. C. Iverson, K. A. Toulouse, and D. W. Mahoney Effects of Methylene Blue Stabilizer on In Vitro Viability and Chemotaxis of 99mTc-Exametazime-Labeled Leukocytes J. Nucl. Med., July 1, 2002; 43(7): 928 - 932. [Abstract] [Full Text] [PDF]