HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ohira, H. Articles by Satomi, S. Search for Related Content PubMed PubMed Citation Articles by Ohira, H. Articles by Satomi, S.

Comparison of Intratumoral Distribution of ^99mTc-MIBI and Deoxyglucose in Mouse Breast Cancer Models

Second Department of Surgery, Tohoku University School of Medicine, Sendai
Department of Nuclear Medicine and Radiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan

Correspondence: For correspondence or reprints contact: Kazuo Kubota, MD, Department of Nuclear Medicine and Radiology, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.

ABSTRACT

The aims of this study were to evaluate the distribution of ^99mTc-methoxyisobutylisonitrile (MIBI) in 3 animal models of breast cancer, the effect of radiotherapy on ^99mTc-MIBI uptake, and the relationship between uptake and microvessel density. Methods: We used syngeneic, subcutaneously transplanted FM3A, MM48, and Ehrlich mouse breast cancer. ^99mTc-MIBI and FDG were injected intravenously, and tumor uptake was measured 30 min later. Double-tracer macroautoradiography (ARG) images were prepared with ^99mTc-MIBI and 2-deoxy-D-[1-¹⁴C]-glucose (¹⁴C-DG), analyzed quantitatively, and compared with histology. The radiotherapeutic effects of 20 Gy x-ray irradiation were monitored by measuring tumor volume, tumor uptake, and ARG findings using ^99mTc-MIBI and FDG in FM3A tumors. Microvessel density was quantified by immunohistochemical staining for CD34 and compared with ARG using ^99mTc-MIBI in FM3A tumors. Results: FM3A, MM48, and Ehrlich tumors showed different growth rates and radiosensitivities. Uptake of FDG, but not of ^99mTc-MIBI, correlated significantly with growth rates. Compared with ^99mC-DG, ^99mTc-MIBI accumulated more in cancer cells and less in infiltrating fibroblasts and macrophages in all tumor models. Irradiation significantly decreased ^99mTc-MIBI uptake, but a rapid increase was noted at recurrence on day 7. Changes in FDG uptake were not significant at recurrence. Microvessel density in tumor tissue correlated significantly with ^99mTc-MIBI uptake on ARG. Conclusion: Accumulation of ^99mTc-MIBI in cancer cells is preferential and can be used as a sensitive marker to examine the response to radiotherapy. Angiogenesis seems to enhance accumulation of ^99mTc-MIBI in tumors. These characteristics may be favorable for tumor imaging using ^99mTc-MIBI.

Key Words: ^99mTc-methoxyisobutylisonitrile • deoxyglucose • breast cancer • autoradiography • microvessel

This article has been cited by other articles:

				G J R Cook Oncological molecular imaging: nuclear medicine techniques Br. J. Radiol., December 1, 2003; 76(suppl_2): S152 - S158. [Full Text] [PDF]