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Uptake of ^99mTc-MIBI and ^99mTc-Tetrofosmin into Malignant Versus Nonmalignant Breast Cell Lines

Department of Nuclear Medicine and Clinical Division of Oncology, Department of Internal Medicine I, University Hospital, Vienna, Austria

Correspondence: For correspondence or reprints contact: Margarida Rodrigues, MD, Department of Nuclear Medicine, University Hospital, Waehrinoer Guertel 18-20, A-1090 Vienna, Austria.

ABSTRACT

The kinetics and cellular uptake of ^99mTc-2-hexakis 2-methoxyisobutyl-isonitrile (MIBI) and ^99mTc-1,2-bis[bis(2-ethoxyethyl)phosphino]ethane (tetrofosmin) into malignant versus nonmalignant human breast cell lines were investigated and compared. Methods: At specific intervals after incubation at 37°C and 22°C with ^99mTc-MIBI or ^99mTc-tetrofosmin, the uptake characteristics of radiotracers into human adenocarcinoma breast cell lines MCF-7 and SK-BR-3 and human breast, nontumor cell line HBL-100 were assessed. Results: The uptake of ^99mTc-MIBI and ^99mTc-tetrofosmin was lower at an incubation temperature of 22°C than that at 37°C in the 3 cell lines. In MCF-7 and in SK-BR-3 cells the uptake of ^99mTc-MIBI was significantly higher than the uptake of ^99mTc-tetrofosmin. The uptake of ^99mTc-MIBI was significantly higher into MCF-7 and SK-BR-3 cells than that into HBL-100 cells. In comparison with HBL-100 cells, uptake of ^99mTc-tetrofosmin into SK-BR-3 cells was significantly higher, whereas uptake into MCF-7 cells was similar. Conclusion: In vitro data suggest that ^99mTc-MIBI may be a better tracer than ^99mTc-tetrofosmin for discrimination between malignant and nonmalignant breast disease.

Key Words: ^99mTc-MIBI • ^99mTc-tetrofosmin • breast tumors

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