HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fogarasi, M. Articles by Rusckowski, M. Search for Related Content PubMed PubMed Citation Articles by Fogarasi, M. Articles by Rusckowski, M.

Pretargeting of Bacterial Endocarditis in Rats with Streptavidin and ¹¹¹In-Labeled Biotin

Departments of Nuclear Medicine and Pathology, University of Massachusetts Medical Center, Worcester, Massachusetts

Correspondence: For correspondence or reprints contact: Mary Rusckowski, PhD, Department of Nuclear Medicine, University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA 01655.

ABSTRACT

A radioimaging approach for the detection of endocarditis has been investigated using two-step pretargeting with streptavidin and radiolabeled biotin. Methods: Hemodynamic alterations within the rat heart were induced by placing an in-dwelling catheter into the left ventricle through the aortic valves. The animals were subsequently infected with Staphylococcus aureus through a tail vein. After an incubation period, rats were first injected with streptavidin and, 2 h later, with ¹¹¹In-labeled ethylenediaminetetraacetic acid-biotin. Whole-body gamma camera images were taken 4–5 h postinjection of the radiolabeled biotin. Control animals consisted of catheterized but uninfected, infected but uncatheterized and normal untreated rats. As a further control, the labeled biotin was administered to a study animal without the preadministration of streptavidin. Results: Histology showed typical endocarditic changes in the hearts of study animals with massive deposition of gram-positive cocci. Catheterized but uninfected animals showed alterations corresponding to nonbacterial thrombotic endocarditis. Macroautoradiography showed accumulation of radiolabel in the endocarditic vegetations of study animals. Whole-body gamma camera images showed important cardiac uptake in 7 of 8 catheterized and infected animals and in 3 of 6 catheterized but uninfected animals. Normal rats and those infected but not catheterized showed negative results by histology, autoradiography and imaging. The percent uptake of the injected dose in the heart was 0.20 (SD = 0.13) in catheterized and infected animals, 0.12 (SD = 0.10) in catheterized but uninfected animals, 0.10 (SD = 0.04) in infected but uncatheterized animals and 0.04 (SD = 0.01) in normal control animals. Conclusion: The two-step pretargeting approach using streptavidin and ¹¹¹In-labeled biotin was used successfully to detect S. aureus-induced bacterial endocarditis in rats.

Key Words: endocarditis • streptavidin • biotin • pretargeting

This article has been cited by other articles:

				M. Rusckowski, S. Gupta, G. Liu, S. Dou, and D. J Hnatowich Investigations of a 99mTc-Labeled Bacteriophage as a Potential Infection-Specific Imaging Agent J. Nucl. Med., July 1, 2004; 45(7): 1201 - 1208. [Abstract] [Full Text] [PDF]

				G. Liu, K. Mang'era, N. Liu, S. Gupta, M. Rusckowski, and D. J. Hnatowich Tumor Pretargeting in Mice Using 99mTc-Labeled Morpholino, a DNA Analog J. Nucl. Med., March 1, 2002; 43(3): 384 - 391. [Abstract] [Full Text] [PDF]