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Direct Labeling of Macroaggregated Albumin with Indium-111-Chloride Using Acetate Buffer

Department of Nuclear Medicine, Gunma University School of Medicine, Gunma
Central Research Laboratory, Nihon Medi-physics Co., Ltd., Chiba, Japan

Correspondence: For correspondence contact: Naoyuki Watanabe, MD, Department of Nuclear Medicine, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi-shi, Gunma 371, Japan.

ABSTRACT

Indium-111-labeled macroaggregated albumin (MAA) would be suitable for combined pulmonary perfusion and ventilation scan using a ^99mTc ventilation agent. Methods: MAA suspended in 0.1 M sodium acetate buffer, pH 5.8, was incubated with ¹¹¹In-chloride for 30 min at room temperature. An in vitro study of the obtained ¹¹¹In-MAA was performed for labeling efficiency and stability in human normal serum. The ¹¹¹In-MAA was intravenously injected into normal mice, and the biodistribution was studied at 15 and 180 min post injection. A gamma camera image was obtained at 15 min after injection. Results: MAA was directly and stably labeled with ¹¹¹In-chloride, and the labeling efficiency of the preparation was more than 96%. More than 90% of the administered ¹¹¹In-MAA was caught in the murine lung. The scintigraphy with ¹¹¹In-MAA showed a clearly visualized murine lung. Conclusion: Indium-111-MAA can be conveniently prepared by direct labeling at room temperature. It provides an alternative perfusion tracer for combined perfusion-ventilation imaging.

Key Words: indium-111-MAA • pulmonary perfusion agent

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