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The First Department of Internal Medicine, Tohoku University School of Medicine
Cyclotron and Radioisotope Center, Tohoku University, Sendai, Japan
Correspondence: For correspondence or reprints contact: Kunio Shirato MD, First Department of Internal Medicine, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-77, Japan.
ABSTRACT
During acute regional ischemia, a "border zone" exists where the spatial distributions of blood flow and substrate uptake show gradual changes. We investigated the relationship between blood flow and glucose uptake in the border zone during acute regional ischemia. Methods: Newly developed quantitative autora-diography using imaging plates and two long-lived radioisotopes was applied to rat hearts subjected to 30 min of left coronary arteryocclusion. Blood flow, glucose uptake and fatty acid uptake was assessed with 4-[N-methyl-14C]iodoantipyrine, 2-deoxy-D-[1-3H]glucose (3H-DG) and ß-methyl[1-14C]heptadecanoic acid (14C-BMHDA), respectively. Results: In rats showing 3H-DG uptake in the normally perfused area (Norm) of 254 ± 96 Bq/mg (high-DG) and 56 ± 20 Bq/mg (low-DG) (n = 4 for each), 3H-DG uptake in the border zone was 148 ± 52 Bq/mg and 58 ±15 Bq/mg (p < 0.05 high- versus low-DG), respectively. The relationship between blood flow and 3H-DG uptake in the border zone was altered by the different 3H-DG uptake levels in Norm. In high-DG, 3H-DG uptake in the border zone was reduced significantly according to the decreasein the percentage of blood flow. However, in low-DG, no significant differences in 3H-DG uptake were found among the regions in the border zone with different levels of the percentage of blood flow, except in the region with 10%-19% of the percentage of blood flow. In the border zone, the percentage of 3H-DG uptake per unit bloodflow normalized to that in Norm increased according to the decrease in the percentage of blood flow, and this increase was steeper in low-DG than in high-DG (p < 0.0005). The percentage of 14C-BMHDA uptake was lower than the percentage of 3H-DG uptake (27 ± 3% versus 78 ± 18% of that in Norm, p < 0.0005) in the peripheral ischemie area. Conclusion: The relationship between blood flow and glucose uptake in the ischemie border zone was altered by the different glucose uptake levels in Norm. Glucose uptake in the border zone was higher in rats with higher glucose uptake levels in Norm, suggesting that glucose uptake in the border zone stimulated by ischemia can be accelerated still more by humoral factors.
Key Words: regional myocardial ischemia • myocardial glucose metabolism • myocardial fatty acid metabolism • imaging plate • double-tracer autoradiography
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