HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yamane, Y. Articles by Shirato, K. Search for Related Content PubMed PubMed Citation Articles by Yamane, Y. Articles by Shirato, K.

Quantitative Double-Tracer Autoradiography with Tritium and Carbon-14 Using Imaging Plates: Application to Myocardial Metabolic Studies in Rats

The First Department of Internal Medicine, School of Medicine and Cyclotron and Radioisotope Center, Tohoku University, Sendai, Japan

Correspondence: For correspondence or reprints contact: Nobumasa Ishide, MD, Professor, The First Department of Internal Medicine, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 960-77, Japan.

ABSTRACT

A system for ³H- and ¹⁴C-labeled macroautoradiography was developed that is able to quantify the tissue radioactivity of two tracers using imaging plates. Methods: Discrimination between electrons emitted from ³H and ¹⁴C is possible on the basis of their different energy distributions. The general use imaging plate with a protective layer detects ¹⁴C radioactivity, but it does not detect ³H radioactivity which has a lower energy distribution than ¹⁴C. Recently, a ³H-sensitive imaging plate without a protective layer was developed. The ³H distribution image is obtained by subtracting the UR image from the TR image. For quantification of the tissue radioactivity of ³H and ¹⁴C, we obtained tissue equivalent values (Bq/mg) of commercially available ³H- and ¹⁴C-labeled graded standards using different dilutions of labeled heart paste and liquid scintillation counting. Using the ³H- and ¹⁴C-labeled graded standards, we confirmed the validity of the quantification of the ³H-autoradiographic intensity using this subtraction method. We applied this method to a rat model of acute myocardial ischemia to compare regional myocardial free fatty acid uptake determined by ß-methyl[1-¹⁴C]heptadecanoic acid to glucose uptake determined by 2-deoxy-D-[1-³H]glucose. Results: Free fatty acid uptake was decreased sharply at the ischemic periphery where glucose uptake was preserved. Conclusion: This double-tracer autoradiography with ³H and ¹⁴C which has high sensitivity, a high spatial resolution of 50 µm and superior linearity with a wide dynamic range of 10⁴ to 10⁵ allows accurate quantification of the tissue radioactivity of the two radiopharmaceuticals.

Key Words: imaging plate • double-tracer autoradiography • tritium • carbon-14 • myocardial glucose metabolism

This article has been cited by other articles:

				T. Kaneta, Y. Takai, Y. Kagaya, Y. Yamane, H. Wada, M. Yuki, R. Iwata, M. Tsujitani, S. Takahashi, and S. Yamada Imaging of Ischemic but Viable Myocardium Using a New 18F-Labeled 2-Nitroimidazole Analog, 18F-FRP170 J. Nucl. Med., January 1, 2002; 43(1): 109 - 116. [Abstract] [Full Text] [PDF]

				M. Chida, Y. Kagaya, Y. Imahori, S. Namiuchi, R. Fujii, M. Fukuchi, C. Takahashi, F. Tezuka, T. Ido, and K. Shirato Visualization of Myocardial Phosphoinositide Turnover with 1-[1-11C]-Butyryl-2-Palmitoyl-rac-Glycerol in Rats with Myocardial Infarction J. Nucl. Med., December 1, 2000; 41(12): 2063 - 2068. [Abstract] [Full Text] [PDF]

				Y. Yamane, N. Ishide, Y. Kagaya, D. Takeyama, N. Shiba, M. Chida, T. Nozaki, T. Takahashi, T. Ido, and K. Shirato Heterogeneous fatty acid uptake early after reperfusion in rat hearts Am J Physiol Heart Circ Physiol, March 1, 1998; 274(3): H923 - H929. [Abstract] [Full Text] [PDF]

				Y. Watanabe, H. Kusuoka, K. Fukuchi, T. Fujiwara, and T. Nishimura Contribution of hypoxia to the development of cardiomyopathy in hamsters Cardiovasc Res, August 1, 1997; 35(2): 217 - 222. [Abstract] [Full Text] [PDF]