HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hosono, M. N. Articles by Onoyama, Y. Search for Related Content PubMed PubMed Citation Articles by Hosono, M. N. Articles by Onoyama, Y.

Effect of Hyperthermia on Tumor Uptake of Radiolabeled Anti-Neural Cell Adhesion Molecule Antibody in Small-Cell Lung Cancer Xenografts

Department of Radiology, Osaka City University School of Medicine, Osaka
Department of Nuclear Medicine, Kyoto University Faculty of Medicine, Kyoto
Department of Nuclear Medicine, Gunma University Faculty of Medicine, Maebashi and Laboratory of Chemotherapy, Aichi Cancer Center, Nagoya, Japan

Correspondence: For correspondence and reprints contact: Masako N. Hosono, MD, Department of Radiology, Osaka City University Hospital, Asahi-machi, Abeno-ku, Osaka 545, Japan.

ABSTRACT

This study was performed to examine the effect of hyperthermia on the intratumor accumulation of a monoclonal antibody (Mab) in an animal model. Mab NE15O (IgG1) recognizes the neural cell adhesion molecule (NCAM) expressed by human small-cell lung cancer (SCLC) cells. Methods: Athymic mice inoculated with NCI-H69, an SCLC cell line, received an intravenous injection of ¹²⁵I- and ¹¹¹In-NE150 and the serial changes of the biodistribution were determined. Furthermore, athymic mice beating NCI-H69 were either Sham-treated or treated by a single hyperthermia at 42°C or 43°C for 1 hr, with the tumor-bearing leg in a water bath using pentobarbital anesthesia. Immediately after heating, the mice were given an intravenous injection of radiolabeled NE150, and the biodistribution was examined at 24 and 48 hr. Results: NE150 localized well in the transplanted tumor when compared with a control Mab. In mice treated at 43°C, there was a 1.34- to 1.67-fold increase in the tumor uptake of ¹²⁵I- and ¹¹¹In-NE150 compared to sham-treated mice at both 24 and 48 hr. In addition, a 1.84- to 2.22-fold increase of the tumor-to-blood ratio was demonstrated, since radiolabeled NE150 cleared faster from the circulation in the mice given hyperthermia. A histological study demonstrated the infiltration of neutrophils in the perivascular spaces, indicating an increase of tumor vascular permeability, which might be one of the main reasons for the enhancement of Mab uptake. Conclusion: Hyperthermia seems to be a potential method of achieving an increased tumor accumulation of Mab in the radioimmunotargeting of SCLC.

Key Words: hyperthermia • monoclonal antibody • human small cell lung cancer xenograft • neural cell adhesion molecule

This article has been cited by other articles:

				M. Jain, G. Venkatraman, and S. K. Batra Optimization of Radioimmunotherapy of Solid Tumors: Biological Impediments and Their Modulation Clin. Cancer Res., March 1, 2007; 13(5): 1374 - 1382. [Abstract] [Full Text] [PDF]

				G. Kong, R. D. Braun, and M. W. Dewhirst Characterization of the Effect of Hyperthermia on Nanoparticle Extravasation from Tumor Vasculature Cancer Res., April 1, 2001; 61(7): 3027 - 3032. [Abstract] [Full Text]

				J. F. Gestin, A. Loussouarn, M. Bardies, E. Gautherot, A. Gruaz-Guyon, C. Sai-Maurel, J. Barbet, C. Curtet, J. F. Chatal, and A. Faivre-Chauvet Two-Step Targeting of Xenografted Colon Carcinoma Using a Bispecific Antibody and 188Re-Labeled Bivalent Hapten: Biodistribution and Dosimetry Studies J. Nucl. Med., January 1, 2001; 42(1): 146 - 153. [Abstract] [Full Text] [PDF]

				G. Kong, G. Anyarambhatla, W. P. Petros, R. D. Braun, O. M. Colvin, D. Needham, and M. W. Dewhirst Efficacy of Liposomes and Hyperthermia in a Human Tumor Xenograft Model: Importance of Triggered Drug Release Cancer Res., December 1, 2000; 60(24): 6950 - 6957. [Abstract] [Full Text]

				G. Kong, R. D. Braun, and M. W. Dewhirst Hyperthermia Enables Tumor-specific Nanoparticle Delivery: Effect of Particle Size Cancer Res., August 1, 2000; 60(16): 4440 - 4445. [Abstract] [Full Text]