HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weber, D.A. Articles by Ljunggren, K. Search for Related Content PubMed PubMed Citation Articles by Weber, D.A. Articles by Ljunggren, K.

Pinhole SPECT: An Approach to In Vivo High Resolution SPECT Imaging in Small Laboratory Animals

Medical Department, Brookhaven National Laboratory, Upton, New York
Radiation Physics Department, University of Lund, Lund, Sweden
University Hospital Sestre Milosrdnice, Zagreb, Croatia
Department of Radiology, Health Science Center, State University of New York, Stony Brook, New York

Correspondence: For correspondence or reprints contact: David A. Weber, PhD, Medical Dept., Brookhaven National Laboratory, Upton, New York 11973.

ABSTRACT

The performance of pinhole SPECT and the application of this technology to investigate the localization properties of radiopharmaceuticals in vivo in small laboratory animals are presented. Methods: System sensitivity and spatial resolution measurements of a rotating scintillation camera system are made for a low-energy pinhole collimator equipped with 1.0-, 2.0- and 3.3-mm aperture pinhole inserts. The spatial detail offered by pinhole SPECT for in vivo imaging was investigated in studies of the brain and heart in Fisher 344 rats by administering ²⁰¹TICI, ^99mTc-HMPAO, ^99mTc-DTPA and ^99mTc-MIBI. Image acquisition is performed using a rotating scintillation camera equipped with a pinhole collimator; projection data are acquired in conventional step-and-shoot mode as the camera is rotated 360° around the subject. Pinhole SPECT Images are reconstructed using a modified cone-beam algorithm developed from a two-dimensional fanbeam filtered backprojection algorithm. Results: The reconstructed transaxial resolution of 2.8 mm FWHM and system sensitivity of 0.086 c/s/kBq with the 2.0-mm pinhole collimator aperture provide excellent spatial detail and adequate sensitivity for imaging the regional uptake of the radiopharmaceuticals in tumor, organs and other tissues in small laboratory animals. Conclusion: The resolution properties of pinhole SPECT are superior to those which have been achieved thus far with conventional SPECT or PET imaging technologies. Pinhole SPECT provides an important approach for investigating localization properties of radiopharmaceuticals in vivo.

Key Words: pinhole SPECT • tumor imaging • technetium-99m-DTPA • technetium-99m-MIBI • technetium-99m-HMPAO • thallium-201-chloride

This article has been cited by other articles:

				R. Accorsi Brain Single-Photon Emission CT Physics Principles AJNR Am. J. Neuroradiol., August 1, 2008; 29(7): 1247 - 1256. [Abstract] [Full Text] [PDF]

				Y. Miao, K. Benwell, and T. P. Quinn 99mTc- and 111In-Labeled {alpha}-Melanocyte-Stimulating Hormone Peptides as Imaging Probes for Primary and Pulmonary Metastatic Melanoma Detection J. Nucl. Med., January 1, 2007; 48(1): 73 - 80. [Abstract] [Full Text] [PDF]

				E. R. McVeigh Emerging Imaging Techniques Circ. Res., April 14, 2006; 98(7): 879 - 886. [Abstract] [Full Text] [PDF]

				M. C. Wu, D.-W. Gao, R. E. Sievers, R. J. Lee, B. H. Hasegawa, and M. W. Dae Pinhole single-photon emission computed tomography for myocardial perfusion imaging of mice J. Am. Coll. Cardiol., August 6, 2003; 42(3): 576 - 582. [Abstract] [Full Text] [PDF]

				F. Pain, L. Besret, F. Vaufrey, M.-C. Gregoire, L. Pinot, P. Gervais, L. Ploux, G. Bloch, R. Mastrippolito, P. Laniece, et al. In vivo quantification of localized neuronal activation and inhibition in the rat brain using a dedicated high temporal-resolution beta +-sensitive microprobe PNAS, August 6, 2002; 99(16): 10807 - 10812. [Abstract] [Full Text] [PDF]

				T. Kudo, K. Fukuchi, A. J. Annala, A. F. Chatziioannou, V. Allada, M. Dahlbom, Y.-C. Tai, M. Inubushi, S.-C. Huang, S. R. Cherry, et al. Noninvasive Measurement of Myocardial Activity Concentrations and Perfusion Defect Sizes in Rats With a New Small-Animal Positron Emission Tomograph Circulation, July 2, 2002; 106(1): 118 - 123. [Abstract] [Full Text] [PDF]

				Z. Liu, G. A. Kastis, G. D. Stevenson, H. H. Barrett, L. R. Furenlid, M. A. Kupinski, D. D. Patton, and D. W. Wilson Quantitative Analysis of Acute Myocardial Infarct in Rat Hearts with Ischemia-Reperfusion Using a High-Resolution Stationary SPECT System J. Nucl. Med., July 1, 2002; 43(7): 933 - 939. [Abstract] [Full Text] [PDF]

				J. B.A. Habraken, K. de Bruin, M. Shehata, J. Booij, R. Bennink, B. L.F. van Eck Smit, and E. Busemann Sokole Evaluation of High-Resolution Pinhole SPECT Using a Small Rotating Animal J. Nucl. Med., December 1, 2001; 42(12): 1863 - 1869. [Abstract] [Full Text] [PDF]

				R. Weissleder and U. Mahmood Molecular Imaging Radiology, May 1, 2001; 219(2): 316 - 333. [Abstract] [Full Text]