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Effect of Hypoxia on the Accumulation of Technetium-99m-Glucarate and Technetium-99m-Gluconate by Chinese Hamster Ovary Cells In Vitro

Divisions of Nuclear Medicine and Experimental Therapeutics, Ontario Cancer Institute/Princess Margaret Hospital
Departments of Pharmacy, Medicine and Medical Biophysics, University of Toronto, Toronto, Ontario, Canada

Correspondence: For correspondence or reprints contact: J.R. Ballinger, PhD, Department of Nuclear Medicine. Princess Margaret Hospital, 500 Sherbourne Street, Toronto, Ontario, Canada, M4X 1K9.

ABSTRACT

The accumulation of ^99mTc-glucarate, an agent recently reported to localize in acutely infarcted myocardium, zones of acute cerebral injury and tumors, has been compared with ^99mTc-gluconate in an in vitro system of cultured Chinese hamster ovary fibroblasts. The effects on accumulation of hypoxia and competition with fructose have been studied. Both labeled glucose analogs showed a two- to threefold enhanced accumulation in hypoxic cells relative to aerobic cells. No such enhanced accumulation under hypoxia was observed for the nonsugar tracers pertechnetate and ^99mTc-DTPA. The presence of 20 mM fructose reduced the accumulation of ^99mTc-glucarate by 30% (p 0.067) and ^99mTc-gluconate by 40% (p < 0.05) in hypoxic cells, but had no significant effect in aerobic cells. These results suggest that both compounds at least partially share a common mechanism of uptake and/or accumulation with fructose.