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Localization of Indium-111-Immunoglobulin G, Technetium-99m-Immunoglobulin G and Indium-111-Labeled White Blood Cells at Sites of Acute Bacterial Infection in Rabbits

Division of Nuclear Medicine of the Department of Radiology and The Clinical Investigation Unit of the Medical Service, Massachusetts General Hospital
Department of Radiology and Medicine, Harvard Medical School, Boston, Massachusetts
R. W. Johnson Pharmaceutical Research Institute, Spring House, Pennsylvania

Correspondence: For correspondence and reprints contact: Dr. Alan J. Fischman, Div. of Nuclear Medicine, Massachusetts General Hospital, Boston, MA 02114.

ABSTRACT

Biodistribution and infection imaging properties of ¹¹¹In-DTPA-IgG, ^99mTc-hydrazino nicotinamide-IgG and ¹¹¹In-WBC were compared in rabbits with E. coli infection. Groups of six rabbits were injected with 10 mCi of ^99mTc-IgG plus 0.5 mCi of ¹¹¹In-IgG or 1 mCi of ^99mTc-IgG plus 0.05 mCi of ¹¹¹In-WBC. At 4–5 and 18–20 hr, dual photon scintigrams were acquired. At both times, the distributions of ^99mTc and ¹¹¹In-IgG were nearly identical. The sites of infection were well visualized with all three radiopharmaceuticals. In the early images, the target-to-background ratios (T/B) for ¹¹¹In and ^99mTc-IgG determined by ROI analysis were 1.95 ± 0.26 and 2.57 ± 0.38 (p = NS). In the delayed images, the T/B ratios increased (p < 0.01) to 3.56 ± 0.49 and 4.90 ± 0.98. At both times, the T/B ratios for ¹¹¹In-WBC were higher (p < 0.01); 4.17 ± 0.78 at 4–5 hr and 8.52 ± 1.52 at 18–20 hr. These results indicate that all three agents yield excellent images of infection sites. Although ¹¹¹In-WBC had higher T/B ratios, the ease of preparation of the radiolabeled proteins makes them attractive alternatives for infection imaging.