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Comparison of Oxine and Tropolone Methods for Labeling Human Platelets with Indium-111

MRC Blood Platelet Research Unit and Department of Biophysics, University of the Orange Free State, Bloemfontein, South Africa

Correspondence: For reprints contact: Professor A. du P. Heyns, MD, Director, Blood Platelet Research Unit, PO Box 339 (G2), Bloemfontein 9300, South Africa.

ABSTRACT

The effect of the chelates oxine and tropolone, used to label platelets, on the kinetics of indium-111-(¹¹¹In) labeled platelets was studied in twelve normal human subjects. Autologous platelets were labeled either in saline with ¹¹¹In-oxine or in plasma with ¹¹¹In-tropolone. Mean platelet lifespan was estimated by fitting the disappearance curve of platelets from the circulation to the multiple hit and other mathematical models. The in vivo distribution of platelets was quantitatively imaged with a scintillation camera. The in vivo recovery of ¹¹¹In-oxine and ¹¹¹In-tropolone did not differ, and the mean platelet lifespan was also similar (¹¹¹In-oxine: 230 ± 29 hr; ¹¹¹In-tropolone: 226 ± 13 hr). At equilibrium (90 min after reinjection of labeled platelets) and at the end of platelet lifespan, ¹¹¹In-oxine and ¹¹¹In-tropolone radioactivities in the spleen and liver were similar. These results demonstrate that the results of kinetics measured with ¹¹¹In-oxine or ¹¹¹In-tropolone do not differ significantly.

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