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Localization of Pulmonary Human Sarcoma Xenografts in Athymic Nude Mice with Indium-111-labeled Monoclonal Antibodies

Division of Surgical Oncology, Department of Surgery, University of Illinois College of Medicine at Chicago
Cook County Hospital
West Side Veterans Administration Hospital
Hektoen Institute for Medical Research
Division of Nuclear Medicine, Michael Reese Hospital, Chicago, Illinois
Argonne National Laboratory, Argonne, Illinois
National Institutes of Health, Bethesda, Maryland

Correspondence: For reprints contact: John A. Greager, MD, Division of Surgical Oncology, Department of Surgery, University of Illinois College of Medicine, 840 South Wood St., Chicago, IL 60612.

ABSTRACT

In order to study localization of metastatic tumors with a radiolabeled monoclonal antibody, a pulmonary metastases model was devised in athymic mice. Metastatic pulmonary sarcoma colonies were verified by histological examination. A murine monoclonal antibody (MAb 19–24) directed against a human sarcoma antigen was labeled with indium-111 (¹¹¹ln) by use of the linker 1-(p-isothiocyanatobenzyl)-diethylenetnaminepentaacetic acid (SCN-Bz-DTPA). MAb P3 was similarly labeled as a negative control. In the group given MAb 19–24, the percent injected dose per gram lung tissue bearing tumor colonies (30.1%, 29.6%, and 27.7% on Days 1, 2, and 3, respectively) was significantly (p < 0.05) higher than in those receiving MAb P3. Hepatic activities of both ¹¹¹ln-MAb 19–24 and ¹¹¹ln-MAb P3 were low. The lungs with tumor colonies demonstrated clearest images on Day 3. The specific binding of ¹¹¹ln-SCN-Bz-DTPA-labeled MAb 19–24 to pulmonary xenografts without appreciable liver uptake indicates that it maybe useful in the clinical localization of pulmonic metastatic lesions.