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In Vivo Traffic of Indium-111-Oxine Labeled Human Lymphocytes Collected by Automated Apheresis

Department of Transfusion Medicine and Department of Nuclear Medicine, National Institutes of Health, Bethesda, Maryland

Correspondence: For reprints contact: Elizabeth J. Read, MD, Department of Transfusion Medicine, Bldg 10, Rm. 5D-56, National Institutes of Health, Bethesda, MD 20892.

ABSTRACT

The in vivo traffic patterns of autologous lymphocytes were studied in five normal human volunteers using lymphocytes obtained by automated apheresis, separated on Ficoll-Hypaque gradients, and labeled ex vivo with ¹¹¹In-oxine. Final lymphocyte infusions contained 1.8–3.1 x 10⁹ cells and 270–390 µCi (9.99–14.43 MBq) ¹¹¹In, or 11–17 µCi (0.41–0.63 MBq) per 10⁸ lymphocytes. Gamma imaging showed transient lung uptake and significant retention of radioactivity in the liver and spleen. Progressive uptake of activity in normal, nonpalpable axillary and inguinal lymph nodes was seen from 24 to 96 hr. Accumulation of radioactivity also was demonstrated at the forearm skin test site, as well as in its associated epitrochlear and axillary lymph nodes, in a subject who had been tested for delayed hypersensitivity with tetanus toxoid. Indium-111-oxine labale human lymphocytes may provide a useful tool for future studies of normal and abnormal lymphocyte traffic.

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