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Radiolabeling of Erythrocytes with Technetium-99m: Role of Band-3 Protein in the Transport of Pertechnetate Across the Cell Membrane

Nuclear Medicine Division, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Correspondence: For reprints contact: Carlos A. Rabito, MD, PhD, Nuclear Medicine Division, Department of Radiology, Massachusetts General Hospital, Boston, MA 02114.

ABSTRACT

This study analyzes the transport characteristics of thepertechnetate anion across membranes of human erythrocytesduring labeling with technetium-99m (^99mTc).Transport of this anion is inhibited after incubation at lowtemperature, indicating the involvement of a process withhigh activation energy. Transport is also decreased by twowell-known inhibitors of the band-3 anion transport systemand is not affected by inhibition of the Na/K/Cl co-transportsystem. From these results we conclude that, in the processof labeling red blood cells with ^99mTc, the pertechnetateanion may reach the interior of the erythrocyte through theband-3 anion transport system.