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Preparation and Evaluation of a ^99mTc-SnF₂ Colloid Kit for Leukocyte Labeling

Department of Radiology, Virginia Commonwealth University Health Sciences Division
Medical College of Virginia, Richmond, Virginia

Correspondence: For reprints contact: Jerry I. Hirsch, PharmD, Box 1, MCV Station, Richmond, VA 23298-0001.

ABSTRACT

Stannous fluoride colloid (SFC) kits for instant radiolabeling with ^99mTc were prepared and evaluated for suitability as a leukocyte radiolabeling agent. Technetium-99m labeling for kits stored at –15°C for up to 3 mo was >95% as determined by instant thin layer chromatography while colloid particles of 1–3 µm were measured by electron microscope for these preparations. Canine leukocyte preparations labeled with [^99mTc]SFC and characterized by triple density gradients of metrizamide in plasma demonstrated an 83% leukocyte association. Analysis of labeled cell preparation for up to 3 hr demonstrated label stability. Labeled leukocytes, when readministered in normal dogs, demonstrated bi-exponential blood clearance with uptake and subsequent clearance from lung. There was increasing uptake of labeled leukocytes by the liver until steady state was achieved. Furthermore, when whole blood samples were analyzed by the triple density gradient method, an increasing monocyte-to-granulocyte ratio was observed to occur with time. By 3 hr 95% of the whole blood activity was associated with the leukocyte fraction. Dogs in which a 24-hr sterile abscess was created demonstrated elevated blood-pool activity as compared to control with localization of the labeled cells at inflammatory sites within 3 hr following cell readministration.

FOOTNOTES

This study was presented in part at the 35th Annual Meeting of the Society of Nuclear Medicine, San Francisco, CA, June 14–17, 1988.