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Radionuclide Imaging of Experimental Atherosclerosis with Nonspecific Polyclonal Immunoglobulin G

Division of Nuclear Medicine of the Department of Radiology and the Infectious Disease and Cardiac Units of the Medical Service of the Massachusetts General Hospital and the Departments of Radiology and Medicine, Harvard Medical School, Boston, Massachusetts
Ortho Biotech Imaging Products, Washingtons Crossing, New Jersey

Correspondence: For reprints contact: H. William Strauss, MD, Div. of Nuclear Medicine, Dept. of Radiology, Massachusetts General Hospital, 32 Fruit St., Boston, MA 02114.

ABSTRACT

The utility of nonspecific polyclonal IgG for external imaging of experimental atherosclerosis was tested in a series of rabbits after balloon catheter deendothelialization of the abdominal aorta. Following injection of ¹¹¹In-IgG, ¹¹¹In-Fc, or ¹¹¹In-Fab serial images were recorded. In addition, several animals received ¹²⁵I-low density lipoproteins [¹²⁵I-LDL], or ¹²⁵I human serum albumin [¹²⁵I-HSA] as positive and negative controls. Forty-eight hours after injection of the radiolabeled proteins, the aortas were removed, divided into abdominal and thoracic regions, counted, and autoradiographed. The images acquired after injection of ¹¹¹In-IgG and ¹¹¹In-Fc, showed dear focal accumulation of radioactivity in the healing abdominal aorta. In contrast, the images obtained after injectionof ¹¹¹In-Fab did not show focal radionuclide accumulation. For ¹¹¹In-IgG and ¹¹¹In-Fc there were three to six times as many counts in the abdominal as in the thoracic aorta, while for ¹¹¹In-Fab and ¹²⁵I HSA, the abdominal and thoracic counts were nearly equal. The results suggest that radiolabeled IgG and Fc can be used to image experimental atherosclerosis.

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