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Pre-Targeted Immunoscintigraphy of Murine Tumors with Indium-111-Labeled Bifunctional Haptens

Nuclear Medicine Service, Veterans Administration Medical Center, Palo Alto
Stanford University School of Medicine, Stanford
Department of Chemistry, University of California, Davis, California

Correspondence: For reprints contact: David A. Goodwin, Nuclear Medicine Service (115), Veterans Administration Medical Center, 3801 Miranda Ave., Palo Alto, CA 94304

ABSTRACT

A method of tumor imaging utilizing the nonspecific accumulation of antibody through leaky capillaries is described, in which the antibody and the radiolabel are administered separately. Nonradioactive antibody is given first (pre-targeted), and allowed adequate time to reach maximum tumor concentration. Depending on the antibody, this may take several days. At the time of maximum tumor concentration of nonradioactive antibody, the blood is quickly cleared of excess circulating nonradioactive antibody using a special i.v. "chase". The radiolabel then is given and imaging done in 1 to 3 hr. The use of short lived tracers (hours) to image antibodies that localize slowly (days) in-vivo is made possible by this method.

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