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Hexakis (2-Methoxy Isobutylisonitrile) Technetium-99m and Thallium-201 Chloride: Uptake and Release in Cultured Myocardial Cells

Centre Jean Perrin and INSERM U 195, Faculté de Médecine, Clermont-Ferrand, France

Correspondence: For reprints contact: Jean C. Maublant, MD, Div. of Nuclear Medicine, Centre Jean Perrin, 63011—Clermont-Ferrand, France.

ABSTRACT

Hexakis (2-methoxy isobutylisonitrile) technetium-99m ([^99mTc]MIBI), a new tracer of myocardial blood flow, was compared with ²⁰¹Tl Cl in cultures of myocardial cells of newborn rats. The kinetics of uptake and release of both tracers were assessed in basal conditions and in the presence of 5 mM cyanide, an inhibitor of the respiratory chain, 0.1 mM iodoacetate, an inhibitor of glycolysis, 10 µM ouabain, an inhibitor of the Na-K ATPase, or with various pH values. The amplitude and frequency of contractions of the cells were also monitored in the same conditions. Results show that the washin and washout kinetics of [^99mTc]MIBI are slower than ²⁰¹Tl (T) of the washout curves were, respectively, of 28 min and 6 min). The kinetics of release of both tracers were not influenced by any of the inhibitors. There was a strong effect of the pH on the ²⁰¹Tl uptake only. Moreover ²⁰¹Tl uptake was decreased by 34% in the presence of cyanide plus iodoacetate. Otherwise the uptakes of ²⁰¹Tl and [^99mTc]MIBI were not decreased by any of the drugs. The cellular contractility was significantly diminished by cyanide and it was abolished by cyanide plus iodoacetate. It is concluded that (a) impaired contractility can be associated with normal ²⁰¹Tl and [^99mTc]MIBI kinetics in myocardial cells in culture, (b) that ²⁰¹Tl uptake may depend on the level of ATP devoted to the maintenance of membrane integrity, (c) that [^99mTc]MIBI shows slower kinetics but is less sensitive to metabolic inhibitors than ²⁰¹Tl.

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