HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chan, S. M. Articles by Duray, P. Search for Related Content PubMed PubMed Citation Articles by Chan, S. M. Articles by Duray, P.

Inhibition of Gallium-67 Uptake in Melanoma by an Anti-Human Transferrin Receptor Monoclonal Antibody

Section of Nuclear Medicine, Department of Radiology, and Department of Pathology, Yale University School of Medicine, New Haven, Connecticut

Correspondence: For reprints contact: Paul B. Hoffer, MD, Sect, of Nuclear Medicine, Dept. of Radiology, Yale Univ. School of Medicine, P.O. Box 3333, New Haven, CT 06510.

ABSTRACT

The effect of an anti-human transferrin receptor (anti-TFR) monoclonal antibody (MoAb), designated B3/25, and an anti-melanoma antibody, designated 96.5, on the uptake of gallium-67 (⁶⁷Ga) by tumor was studied. Three groups of six athymic mice bearing a human melanoma were injected via tail vein with (a) 0.55 mg human serum albumin (HSA) (control group), (b) 0.5 mg MoAb B3/25 + 0.55 mg HSA, and (c) 0.5 mg MoAb 96.5 + 0.55 mg HSA, respectively. Twenty-four hours later, each mouse was given an intravenous dose of 5 µCi [⁶⁷Ga] citrate. Biodistribution of activity (percent injected dose per gram) determined 48 hr after injection of ⁶⁷Ga showed a 75% decrease in tumor uptake in the group of mice that received B3/25 (anti-TFR MoAb) compared with the control group. In contrast, MoAb 96.5 did not show any effect on melanoma uptake of ⁶⁷Ga. Histologic findings suggest that the decreased uptake was not due to cellular damage resulting from binding of B3/25 to TFR. The results of this study strongly suggest the involvement of TFR in the in vivo tumor uptake of⁶⁷Ga.

This article has been cited by other articles:

				T. B. Chaston, D. B. Lovejoy, R. N. Watts, and D. R. Richardson Examination of the Antiproliferative Activity of Iron Chelators: Multiple Cellular Targets and the Different Mechanism of Action of Triapine Compared with Desferrioxamine and the Potent Pyridoxal Isonicotinoyl Hydrazone Analogue 311 Clin. Cancer Res., January 1, 2003; 9(1): 402 - 414. [Abstract] [Full Text] [PDF]