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Technetium-99m Labeling of Murine Monoclonal Antibody Fragments

Summa Medical Corporation, Albuquerque, New Mexico

Correspondence: For reprints contact: P.O. Zamora, PhD, Summa Medical Corp., 4272 Balloon Park Rd. N.E., Albuquerque, NM 87109.

ABSTRACT

F(ab')₂ fragments of several murine monoclonal antibodies have been labeled with ^99mTc by a direct, pretinning method. The fragments were incubated with stannous ions overnight to split disulfide groups—a process which converts dimeric F(ab')₂ to monomeric fragments. The pretinned fragments were then either directly labeled with ^99mTc, frozen for subsequent labeling, or lyophilized to make kits for ^99mTc-labeling at some later date. The ^99mTc-labeled fragments were shown to be stable against transchelation when challenged with ethylenediaminetetraacetic acid, retained immunoreactivity, and were capable of binding to human tumor xenografts in nude mice.

FOOTNOTES

^* Present address: RhoMed, Inc., 1104 Stanford Dr. N.E., Albuquerque, NM 87109.