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Iodination of Monoclonal Antibodies for Diagnosis and Radiotherapy Using a Convenient One Vial Method

Centocor, Malvern, Pennsylvania
The Netherlands Cancer Institute, Amsterdam, The Netherlands

Correspondence: For reprints contact: Vincent R. Zurawski, Jr., Centocor, 244 Great Valley Parkway, Malvern, PA 19355.

ABSTRACT

We have developed a convenient system that can be used to iodinate monoclonal antibodies for diagnosis or therapy. A vial, previously coated with 1,3,4,6-tetrachloro-3a, 6a-diphenyl glycouril (iodogen), is used as a reaction vessel. Iodination and separation of bound and free iodide, using AG1-X8 ion exchange resin, are both accomplished in this vial. We found 90 ± 4% of the iodide which was added was incorporated, respectively, into each of four different monoclonal antibodies evaluated. Approximately 90% of labeled antibody was recovered in each case. The monoclonal antibody OC125 was labeled to specific activities up to 2315 mCi/mg. Immunoreactivities of 82 ± 2% using ¹²⁵I and 66 ± 5% using ¹³¹I were achieved. As the radioiodination is done in one sealed vial and takes <15 min, this procedure is safe and can be performed in any nuclear medicine laboratory. The final product, which is sterile and apyrogenic, is suitable for diagnostic and radiotherapeutic applications.

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