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Gallium-68 Lipophilic Complexes for Labeling Platelets

Donner Laboratory and Lawrence Berkeley Laboratory, University of California, Berkeley, California

Correspondence: For reprints contact: Yukio Yano, BS, Donner Laboratory and Lawrence Berkeley Laboratory, University of California, Berkeley, CA 94720.

ABSTRACT

Generator produced ⁶⁸Ga-labeled platelets could be useful for positron emission tomographic (PET) studies of thrombosis or atheroscierosis. To label platelets with ⁶⁸Ga, we have studied the effects of trace metals in elutions of ⁶⁸Ga from ⁶⁸Ge. Studies were conducted on the formation of lipophilic ⁶⁸Ga complexes 8-hydroxyquinoline, tropolone, and mercaptopyridine-N-oxide(MPO). Parameters such as pH, buffers, concentration of ligand, and stability with time were investigated. High performance liquid chromatography and instant thin layer chromatography were used to quantitate formation of the ⁶⁸Ga complex. Platelets from human, dog, and rabbit plasma were incubated with the ⁶⁸Ga complexes and the percent labeling determined. Accumulation of platelets in the catheter scraped aorta of the rabbit was determined by PET imaging, tissue counting, and autoradiography. Gallium-68 MPO gave 40–60% labeling of rabbit platelets with higher accumulation in the scraped aorta compared to the normal.