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Stoichiometric Tc-99m RBC Labeling using Stable Kit Solutions of Stannous Chloride and EDTA: Concise Communication

Indiana University Medical Center, Indianapolis, Indiana

Correspondence: For reprints contact: Bruce H. Mock, PhD, Div. Nucl. Med., Indiana University Medical Center, UH P-16, 926 West Michigan St., Indianapolis, IN 46223.

ABSTRACT

An in vitro Tc-99m labeling method is described, which utilizes stable stock solutions of stannous chloride and disodium edetate (EDTA). The kit procedure requires as little as 1 ml of patient blood, can be performed in only 15 min, and gives labeling yields in excess of 98%. By using EDTA, the binding capacity of RBCs for technetium is sufficient to produce Tc-99m RBC doses with specific concentrations greater than 50 mCi/ml for first-pass cardiac studies. Scintigrams reveal a slight amount of bladder activity and splenic uptake, but at no time has the thyroid, stomach, or normal bowel been visualized. The predominant 20-hr blood-clearance half-time results in excellent image quality for over 24—an essential property for following intermittent GI bleeding or for performing repeat cardia function studies over a several-hour time interval.