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Sequential Imaging of Indium-111-Labeled Monoclonal Antibody in Human Mammary Tumors Hosted in Nude Mice

Massachusetts General Hospital, Boston, Massachusetts
Research Institute of Scripps Clinic, La Jolla, California
Mallinckrodt Inc., St. Louis, Missouri

Correspondence: For reprints contact: Ban An Khaw, Phd, Cellular and Molecular Research Lab, Jackson 13, Massachusetts General Hospital, Boston, MA 02114.

ABSTRACT

Using a bifunctional chelating agent, indium-111 was attached to a monoclonal antibody 10-3D2, specific for a 126-kilodalton phosphoglycoprotein antigen associated with human mammary carcinoma, and was then used to localize and visualize human mammary tumors hosted in nude mice. Simultaneous tumor concentration of In-111-10-3D2 was eight times greater than that of contgrol I-125-MOPC-21. Uptake of F(ab')₂ and Fab of 10-3D2 was also compared. The scintigrams demonstrated that intact antibody provided the best images. Control In-111-labeled MOPC-21 and plasma did not show specific localization in the tumor. Uptake of In-111-labeled 10-3D2 was also compared in two lines of human mammary tumors, BT-20 and HS-578T. Imaging with 10-3D2 was better for BT-20 than for HS-578T. These studies demonstrated that (a) In-111-10-3D2 can be utilized to image human mammary tumors hosted in nude mice; (b) intact antibody provided the best tumor images, although F(ab')₂ had optimal target-to-background ratios for earlier imaging; and (c) different mammary tumor lines with possibly different concentrations of tumor-associated antigen showed different rates of uptake and apparent saturation with 10-3D2.