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Quatitative Autoradiography with Radiopharmaceuticals, Part 2: Applications in Radiopharmaceutical Research: Concise Communication

Brookhaven National Laboratory, Upton, New York
State University of New York at Stony Brook, New York

Correspondence: For reprints contact: P. Som, Medical Department, Brookhaven National Laboratory, Upton, NY 11973.

ABSTRACT

We describe the application of macroautoradiography, a relatively simple, quantifiable method for the evaluation of positron-emitting and gamma-emitting radiopharmaceuticals. We have investigated the response properties of two types of film to positron (F-18) and negatron (C-14)emitters. Variations in the response of film to increasing film-to-source distance are described, along with the effects of different intensifying screens and mounting tape. Digitization of whole-body autoradiograms (WBARG) in small animals was performed by using a videodensitometry system (videocamera interfaced to a computer). Quantitation was derived from analysis of a series of step-wedge standards that covered the range of radioactivities in the sample. By using a close-up lens on the videocamera, a 2-by 2-cm field is digitized as a 128 x 128 array, each pixel representing 156 x 156 µm. The effect of chlorpromazine (CPZ)on glucose metabolism in mice was studied by giving C-14 2DG followed by CPZ and F-18 FDG in the same animal. Muscle activity decreased and brown-fat activity increased. The high spatial resolution of this technique enables quantification in structures as small as the basal ganglia in mice. The use of dual-nuclide ARG permits each animal to be its own control, which greatly increases the utility of this method.

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