HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Khaw, B. A. Articles by Haber, E. Search for Related Content PubMed PubMed Citation Articles by Khaw, B. A. Articles by Haber, E.

Technetium-99m Labeling of Antibodies to Cardiac Myosin Fab and to Human Fibrinogen

Massachusetts General Hospital, Boston, Massachusetts

Correspondence: For reprints contact: Edgar Haber, MD, Cardiac Unit, Mass. General Hosp., Boston, MA 02114.

ABSTRACT

We have developed a method of labeling biologically active labile macromolecules, such as human fibrinogen (HF) and anticardiac-myosin Fab (AM-Fab), with Tc-99m at neutral pH. This method uses dithionite reduction of pertechnetate and subsequent labeling, to test the method with acid-labile macromolecules. Complexes of diethylene triamine pentaacetic acid with macromolecules such as human fibrinogen (D-HF) and anticardiac-myosin Fab (D-AM-Fab) were labeled and utilized in in vitro and in vivo studies. In biodistribution studies, the Tc-99m D-HF had a two-component blood clearance (half-times 1 hr and 15 hr) and was 80–88% coagulable. The Tc-99m AM-Fab retained its immunoreactivity as tested by affinity chromatography; also during in vivo localization in experimental myocardial infarction. This labeling technique provides an easy and efficient approach to the Tc-99m labeling of other biologically active and acid-labile macromolecules.

This article has been cited by other articles:

				M. Breuer, A. Schutz, M. Engelhardt, C. Hammer, B.M. Kemkes, and M. Breuer Scintigraphy and Immunohistology of Antimyosin-Fab During Graft Rejection Angiology, July 1, 1999; 50(7): 563 - 571. [Abstract] [PDF]