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Indium-111 Tropolone, A New High-Affinity Platelet Label: Preparation and Evaluation of Labeling Parameters

Mayo Clinic and Mayo Foundation, Rochester, Minnesota

Correspondence: For reprints contact: M. K. Dewanjee, PhD, Mayo Clinic, Rochester, MN 55905.

ABSTRACT

Platelets were labeled with a new neutral, lipid-soluble metal complex of indium-111 and tropolone. Unlike oxine, which must be dissolved in ethyl alcohol, tropolone is soluble in isotonic saline. Platelet labeling with in-111 tropolone can be performed in both acid-citrate-dextrose (ACD)-plasma and ACD-saline media within two hours' time. Labeling efficiency has been 80–90% in ACD-saline and 60–70% in the ACD-plasma medium.

Optimum concentrations for the labeling of platelets with in-111 tropolone were 5 µg/ml in ACD-saline and 10 µg/ml in ACD-plasma, using a 15-min incubation at room temperature. A kit formulation for convenient routine preparation of in-111-labeled platelets has been developed. Seven parameters of platelet labeling were studied: concentration of tropolone, citrate, plasma proteins, and calcium ions; also platelet density, temperature, and pH of incubation medium. Their effects on the mechanism of platelet labeling with lipid-soluble tracers are discussed.

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